Quantification of Parathyroid Hormone and its Fragments in Serum by Liquid Chromatography–High-Resolution Mass Spectrometry

Erica M. Fatica, Nicholas E. Larkey, Ravinder J. Singh

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Parathyroid hormone (PTH), an 84-amino acid peptide hormone, is a major regulator of calcium homeostasis. Quantification of PTH in serum is used clinically to investigate calcium imbalances and for monitoring osteodystrophy in patients with renal failure. In addition to intact PTH, several PTH fragments are found in circulation. Recent studies have shown that accurate quantification of PTH fragments may provide valuable clinical information in certain scenarios. In this chapter, a high-resolution mass spectrometry–based method for quantification of PTH (1–84) and its fragments is described. This method involves immunoaffinity capture of intact PTH and PTH-fragments followed by liquid chromatography–high-resolution mass spectrometry (LC–HRMS).

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages365-373
Number of pages9
DOIs
StatePublished - 2022

Publication series

NameMethods in Molecular Biology
Volume2546
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Chronic kidney disease
  • Liquid chromatography–high-resolution mass spectrometry
  • Parathyroid hormone
  • Peptide fragments, Immunopurification
  • PTH1–84

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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