Quantification of GDF11 and Myostatin in Human Aging and Cardiovascular Disease

Marissa J. Schafer; Elizabeth J. Atkinson; Patrick M. Vanderboom; Brian Kotajarvi; Thomas A. White; Matthew M. Moore; Charles J. Bruce; Kevin L. Greason; Rakesh M. Suri; Sundeep Khosla; Jordan D. Miller; H. Robert Bergen; Nathan K. LeBrasseur

doi:10.1016/j.cmet.2016.05.023

Quantification of GDF11 and Myostatin in Human Aging and Cardiovascular Disease

Marissa J. Schafer, Elizabeth J. Atkinson, Patrick M. Vanderboom, Brian Kotajarvi, Thomas A. White, Matthew M. Moore, Charles J. Bruce, Kevin L. Greason, Rakesh M. Suri, Sundeep Khosla, Jordan D. Miller, H. Robert Bergen, Nathan K. LeBrasseur

Research output: Contribution to journal › Article › peer-review

115 Scopus citations

Abstract

Growth and differentiation factor 11 (GDF11) is a transforming growth factor β superfamily member with a controversial role in aging processes. We have developed a highly specific LC-MS/MS assay to quantify GDF11, resolved from its homolog, myostatin (MSTN), based on unique amino acid sequence features. Here, we demonstrate that MSTN, but not GDF11, declines in healthy men throughout aging. Neither GDF11 nor MSTN levels differ as a function of age in healthy women. In an independent cohort of older adults with severe aortic stenosis, we show that individuals with higher GDF11 were more likely to be frail and have diabetes or prior cardiac conditions. Following valve replacement surgery, higher GDF11 at surgical baseline was associated with rehospitalization and multiple adverse events. Cumulatively, our results show that GDF11 levels do not decline throughout aging but are associated with comorbidity, frailty, and greater operative risk in older adults with cardiovascular disease.

Original language	English (US)
Pages (from-to)	1207-1215
Number of pages	9
Journal	Cell Metabolism
Volume	23
Issue number	6
DOIs	https://doi.org/10.1016/j.cmet.2016.05.023
State	Published - Jun 14 2016

ASJC Scopus subject areas

Physiology
Molecular Biology
Cell Biology

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10.1016/j.cmet.2016.05.023

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title = "Quantification of GDF11 and Myostatin in Human Aging and Cardiovascular Disease",

abstract = "Growth and differentiation factor 11 (GDF11) is a transforming growth factor β superfamily member with a controversial role in aging processes. We have developed a highly specific LC-MS/MS assay to quantify GDF11, resolved from its homolog, myostatin (MSTN), based on unique amino acid sequence features. Here, we demonstrate that MSTN, but not GDF11, declines in healthy men throughout aging. Neither GDF11 nor MSTN levels differ as a function of age in healthy women. In an independent cohort of older adults with severe aortic stenosis, we show that individuals with higher GDF11 were more likely to be frail and have diabetes or prior cardiac conditions. Following valve replacement surgery, higher GDF11 at surgical baseline was associated with rehospitalization and multiple adverse events. Cumulatively, our results show that GDF11 levels do not decline throughout aging but are associated with comorbidity, frailty, and greater operative risk in older adults with cardiovascular disease.",

author = "Schafer, {Marissa J.} and Atkinson, {Elizabeth J.} and Vanderboom, {Patrick M.} and Brian Kotajarvi and White, {Thomas A.} and Moore, {Matthew M.} and Bruce, {Charles J.} and Greason, {Kevin L.} and Suri, {Rakesh M.} and Sundeep Khosla and Miller, {Jordan D.} and Bergen, {H. Robert} and LeBrasseur, {Nathan K.}",

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year = "2016",

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doi = "10.1016/j.cmet.2016.05.023",

language = "English (US)",

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AU - Schafer, Marissa J.

AU - Atkinson, Elizabeth J.

AU - Vanderboom, Patrick M.

AU - Kotajarvi, Brian

AU - White, Thomas A.

AU - Moore, Matthew M.

AU - Bruce, Charles J.

AU - Greason, Kevin L.

AU - Suri, Rakesh M.

AU - Khosla, Sundeep

AU - Miller, Jordan D.

AU - Bergen, H. Robert

AU - LeBrasseur, Nathan K.

PY - 2016/6/14

Y1 - 2016/6/14

N2 - Growth and differentiation factor 11 (GDF11) is a transforming growth factor β superfamily member with a controversial role in aging processes. We have developed a highly specific LC-MS/MS assay to quantify GDF11, resolved from its homolog, myostatin (MSTN), based on unique amino acid sequence features. Here, we demonstrate that MSTN, but not GDF11, declines in healthy men throughout aging. Neither GDF11 nor MSTN levels differ as a function of age in healthy women. In an independent cohort of older adults with severe aortic stenosis, we show that individuals with higher GDF11 were more likely to be frail and have diabetes or prior cardiac conditions. Following valve replacement surgery, higher GDF11 at surgical baseline was associated with rehospitalization and multiple adverse events. Cumulatively, our results show that GDF11 levels do not decline throughout aging but are associated with comorbidity, frailty, and greater operative risk in older adults with cardiovascular disease.

AB - Growth and differentiation factor 11 (GDF11) is a transforming growth factor β superfamily member with a controversial role in aging processes. We have developed a highly specific LC-MS/MS assay to quantify GDF11, resolved from its homolog, myostatin (MSTN), based on unique amino acid sequence features. Here, we demonstrate that MSTN, but not GDF11, declines in healthy men throughout aging. Neither GDF11 nor MSTN levels differ as a function of age in healthy women. In an independent cohort of older adults with severe aortic stenosis, we show that individuals with higher GDF11 were more likely to be frail and have diabetes or prior cardiac conditions. Following valve replacement surgery, higher GDF11 at surgical baseline was associated with rehospitalization and multiple adverse events. Cumulatively, our results show that GDF11 levels do not decline throughout aging but are associated with comorbidity, frailty, and greater operative risk in older adults with cardiovascular disease.

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Quantification of GDF11 and Myostatin in Human Aging and Cardiovascular Disease

Abstract

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