TY - JOUR
T1 - Purinergic (P2) receptor-operated calcium entry into rat thyroid cells
AU - Aloj, Salvatore M.
AU - Liguoro, Domenico
AU - Kiang, Juliann G.
AU - Smallridge, Robert C.
PY - 1993/8/31
Y1 - 1993/8/31
N2 - In the epithelial cell line FRT, derived from rat thyroid, extracellular ATP, at a concentration as low as 1×10−7 M, specifically increases cytosolic Ca++ two fold over the basal level of 255±45 nM. A maximum increase of 5 fold over basal is seen at 1×10−5 M ATP. The effect occurs in the absence of any measurable phosphatidyl inositol metabolism and requires the presence of extracellular Ca++ but is independent of extracellular Na+ it is duplicated by ATPγS but not by adenosine, AMP, ADP, AMP-PNP, AMP-CPP, or AMP-PCP. In the presence of the P2-receptor antagonist suramin, the ATP induced Ca++ influx is completely inhibited, whereas Mg++ La+++, and verapamil are ineffective. It appears that the most likely (and unique) mechanism of ATP induced increase of cytosolic Ca++ in FRT cells is an increased influx through the activation of a P2 receptor operated Ca++ channel.
AB - In the epithelial cell line FRT, derived from rat thyroid, extracellular ATP, at a concentration as low as 1×10−7 M, specifically increases cytosolic Ca++ two fold over the basal level of 255±45 nM. A maximum increase of 5 fold over basal is seen at 1×10−5 M ATP. The effect occurs in the absence of any measurable phosphatidyl inositol metabolism and requires the presence of extracellular Ca++ but is independent of extracellular Na+ it is duplicated by ATPγS but not by adenosine, AMP, ADP, AMP-PNP, AMP-CPP, or AMP-PCP. In the presence of the P2-receptor antagonist suramin, the ATP induced Ca++ influx is completely inhibited, whereas Mg++ La+++, and verapamil are ineffective. It appears that the most likely (and unique) mechanism of ATP induced increase of cytosolic Ca++ in FRT cells is an increased influx through the activation of a P2 receptor operated Ca++ channel.
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U2 - 10.1006/bbrc.1993.2000
DO - 10.1006/bbrc.1993.2000
M3 - Article
C2 - 8363591
AN - SCOPUS:0027337372
SN - 0006-291X
VL - 195
SP - 1
EP - 7
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -