Purinergic (P2) receptor-operated calcium entry into rat thyroid cells

Salvatore M. Aloj, Domenico Liguoro, Juliann G. Kiang, Robert C. Smallridge

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

In the epithelial cell line FRT, derived from rat thyroid, extracellular ATP, at a concentration as low as 1×10−7 M, specifically increases cytosolic Ca++ two fold over the basal level of 255±45 nM. A maximum increase of 5 fold over basal is seen at 1×10−5 M ATP. The effect occurs in the absence of any measurable phosphatidyl inositol metabolism and requires the presence of extracellular Ca++ but is independent of extracellular Na+ it is duplicated by ATPγS but not by adenosine, AMP, ADP, AMP-PNP, AMP-CPP, or AMP-PCP. In the presence of the P2-receptor antagonist suramin, the ATP induced Ca++ influx is completely inhibited, whereas Mg++ La+++, and verapamil are ineffective. It appears that the most likely (and unique) mechanism of ATP induced increase of cytosolic Ca++ in FRT cells is an increased influx through the activation of a P2 receptor operated Ca++ channel.

Original languageEnglish (US)
Pages (from-to)1-7
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume195
Issue number1
DOIs
StatePublished - Aug 31 1993

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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