Abstract
Aim: This study was to detect the effects of purified exosome product (PEP) on C28/I2 cells and chondrocytes derived from osteoarthritis patients. Materials & methods: Cell viability and apoptosis assays were used to detect the effect of PEP on cells. qRT-PCR and cell fluorescence assays were used to investigate the potential mechanism of PEP on cell chondrogenesis. Results: PEP was internalized by cells at a fast rate and enhanced cellular proliferation and migration while attenuating apoptosis. These findings reflect the fact that PEP can increase the expression of PCNA and reduce the expression of CASP3/7/9 and BAX. Conclusion: This study suggests an innovative strategy for chondrogenesis that could be applied to osteoarthritis repair in the future.
Original language | English (US) |
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Pages (from-to) | 55-71 |
Number of pages | 17 |
Journal | Regenerative Medicine |
Volume | 18 |
Issue number | 1 |
DOIs | |
State | Published - Jan 1 2023 |
Keywords
- apoptosis
- chondrocytes
- chondrogenesis
- exosomes
ASJC Scopus subject areas
- Biomedical Engineering
- Embryology