@article{2b5d4fa25a7f454f93684ce4e7fa579a,
title = "Purification of rat Schwann cells from cultures of peripheral nerve: an immunoselective method using surfaces coated with anti-immunoglobulin antibodies",
abstract = "We report a method for deriving purified rat Schwann cells by immunoselective removal of fibroblasts. Contaminating fibroblasts labeled with antibody against specific surface marker Thy 1.1 are bound on plastic surfaces coated with a second antibody. The efficacy of the method is demonstrated by flow cytometry and by specific Schwann cell Ran-1 immunofluorescence.",
keywords = "cell culture, panning technique, rat Schwann cell",
author = "Assouline, {Jos{\'e}G G.} and Bosch, {E. Peter} and Ramon Lim",
note = "Funding Information: This work was supportedb y the Departmenotf Neurologya nd by the followingr esearchg rantst o Dr. Lira: NationalS cienceF oundationG rantBNS-8308341, National Cancer Institute Grant CA-31796, Veterans AdministrationM erit Review Award and Career lnvestigatorshiapw ard. We greatly appreciateth e generosityo f Dr. Kay L. Fields for a sampleo f anti-Ran-1s erum.W e thank Dr. Richard G. Lynch for the use of the Nomarski optic-fluorescenmceic roscopeD, r. Andrew Yen for technicaal dviceo n the flow cytometerD, r. Joyce F. Miller for preparingth e pituitarye xtract,a nd Dr. Maurice W. Van Allen for encouragemeonnt this project.",
year = "1983",
month = oct,
day = "31",
doi = "10.1016/0006-8993(83)90953-8",
language = "English (US)",
volume = "277",
pages = "389--392",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier",
number = "2",
}