Purification of rat Schwann cells from cultures of peripheral nerve: an immunoselective method using surfaces coated with anti-immunoglobulin antibodies

JoséG G. Assouline, E. Peter Bosch, Ramon Lim

Research output: Contribution to journalArticle

49 Scopus citations

Abstract

We report a method for deriving purified rat Schwann cells by immunoselective removal of fibroblasts. Contaminating fibroblasts labeled with antibody against specific surface marker Thy 1.1 are bound on plastic surfaces coated with a second antibody. The efficacy of the method is demonstrated by flow cytometry and by specific Schwann cell Ran-1 immunofluorescence.

Original languageEnglish (US)
Pages (from-to)389-392
Number of pages4
JournalBrain Research
Volume277
Issue number2
DOIs
StatePublished - Oct 31 1983

Keywords

  • cell culture
  • panning technique
  • rat Schwann cell

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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