Purification of a glycosaminoglycan-stimulatory lymphokine from supernatants of in vitro-activated human mononuclear cells

Min Tsao, Marina Ferrarini, Christopher H Evans, Arlene Ambro, Theresa L. Whiteside

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Glycosaminoglycans (GAGs), like collagens, are the major component of the connective tissue matrix. Fibroblasts are responsible for the production and maintenance of this matrix. Glycosaminoglycan-stimulatory factor (GAG-SF) from T lymphocytes is a lymphokine that selectively increases GAG synthesis in normal human dermal fibroblasts. Supernatants of mononuclear cells activated with a mitogen, concanavalin A, were used to purify GAG-SF to an apparent homogeneity. The GAG-SF proved to be a glycoprotein with a molecular weight of 67,000 and pI of 5.6. It was not mitogenic to fibroblasts and did not regulate collagen synthesis in confluent fibroblast cultures. The purified factor was distinct from interleukin-1 (IL-1), but it could synergize with IL-1, which was present in unpurified supernatants, in modulating the activation of fibroblasts. The purification and characterization of this lymphokine are important because it is a product of activated lymphocytes and thus may be involved in the immunopathogenesis of several human diseases characterized by the presence of lymphoid infiltrates in the target organ such as autoimmune diseases of the connective tissue, the stromal reactions in human tumors, and the fibrosis associated with chronic rejection of transplanted organs.

Original languageEnglish (US)
Pages (from-to)122-131
Number of pages10
JournalClinical Immunology and Immunopathology
Volume50
Issue number1 PART 1
DOIs
StatePublished - 1989
Externally publishedYes

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Lymphokines
Glycosaminoglycans
Fibroblasts
Interleukin-1
Connective Tissue
Collagen
Concanavalin A
Mitogens
Autoimmune Diseases
Glycoproteins
Fibrosis
Molecular Weight
Maintenance
In Vitro Techniques
Lymphocytes
T-Lymphocytes
Skin
Neoplasms

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Pathology and Forensic Medicine

Cite this

Purification of a glycosaminoglycan-stimulatory lymphokine from supernatants of in vitro-activated human mononuclear cells. / Tsao, Min; Ferrarini, Marina; Evans, Christopher H; Ambro, Arlene; Whiteside, Theresa L.

In: Clinical Immunology and Immunopathology, Vol. 50, No. 1 PART 1, 1989, p. 122-131.

Research output: Contribution to journalArticle

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AU - Whiteside, Theresa L.

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