Purification and immunological quantification of rat liver lysosomal glycosidases

P. C. De Groen, G. D. LeSage, P. S. Tietz, Nicholas F La Russo

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Although lysosomal enzyme activities are known to vary in response to numerous physiological and pharmacological stimuli, the relationship between lysosomal enzyme activity and enzyme concentration has not been systematically studied. Therefore we developed radioimmunoassays for two lysosomal glycosidases in order to determine lysosomal enzyme concentration. β-Galactosidase and β-glucuronidase were purified from rat liver 2780-fold and 1280-fold respectively, by using differential centrifugation, affinity chromatography, ion-exchange chromatography and molecular-sieve chromatography. Polyclonal antibodies to these enzymes were raised in rabbits, and two radioimmunoassays were established. Antibody specificity was shown by: (i) selective immunoprecipitation of enzyme activity; (ii) identical bands of purified enzyme on SDS/polyacrylamide-gel electrophoresis and immunoelectrophoresis; (iii) single immunoreactive peaks in molecular-sieve chromatography experiments. Sensitivities of the assays were such that 15 ng of β-galactosidase and 45 ng of β-glucuronidase decreased the ratio of bound to free radiolabel by 50%; minimal detectable amounts of immunoreactive enzymes were 2 ng and 10 ng respectively. The assays were initially used to assess the effects of physiological perturbations (i.e. fasting and age) on enzyme concentrations in rat liver; these experiments showed that changes in enzyme concentrations do not always correlate with changes in enzyme activities. This represents the first report of radioimmunoassays for lysosomal glycosidases. The results suggest that these radioimmunoassays provide useful technology for the study of regulatory control mechanisms of the concentration of lysosomal glycosidases in mammalian tissues.

Original languageEnglish (US)
Pages (from-to)115-123
Number of pages9
JournalBiochemical Journal
Volume264
Issue number1
StatePublished - 1989

Fingerprint

Glycoside Hydrolases
Liver
Purification
Rats
Enzyme activity
Enzymes
Chromatography
Galactosidases
Radioimmunoassay
Glucuronidase
Molecular sieves
Assays
Affinity chromatography
Centrifugation
Antibodies
Gel Chromatography
Electrophoresis
Ion exchange
Immunoelectrophoresis
Experiments

ASJC Scopus subject areas

  • Biochemistry

Cite this

Purification and immunological quantification of rat liver lysosomal glycosidases. / De Groen, P. C.; LeSage, G. D.; Tietz, P. S.; La Russo, Nicholas F.

In: Biochemical Journal, Vol. 264, No. 1, 1989, p. 115-123.

Research output: Contribution to journalArticle

De Groen, PC, LeSage, GD, Tietz, PS & La Russo, NF 1989, 'Purification and immunological quantification of rat liver lysosomal glycosidases', Biochemical Journal, vol. 264, no. 1, pp. 115-123.
De Groen, P. C. ; LeSage, G. D. ; Tietz, P. S. ; La Russo, Nicholas F. / Purification and immunological quantification of rat liver lysosomal glycosidases. In: Biochemical Journal. 1989 ; Vol. 264, No. 1. pp. 115-123.
@article{78d9919ec084439eb3347550795e84d4,
title = "Purification and immunological quantification of rat liver lysosomal glycosidases",
abstract = "Although lysosomal enzyme activities are known to vary in response to numerous physiological and pharmacological stimuli, the relationship between lysosomal enzyme activity and enzyme concentration has not been systematically studied. Therefore we developed radioimmunoassays for two lysosomal glycosidases in order to determine lysosomal enzyme concentration. β-Galactosidase and β-glucuronidase were purified from rat liver 2780-fold and 1280-fold respectively, by using differential centrifugation, affinity chromatography, ion-exchange chromatography and molecular-sieve chromatography. Polyclonal antibodies to these enzymes were raised in rabbits, and two radioimmunoassays were established. Antibody specificity was shown by: (i) selective immunoprecipitation of enzyme activity; (ii) identical bands of purified enzyme on SDS/polyacrylamide-gel electrophoresis and immunoelectrophoresis; (iii) single immunoreactive peaks in molecular-sieve chromatography experiments. Sensitivities of the assays were such that 15 ng of β-galactosidase and 45 ng of β-glucuronidase decreased the ratio of bound to free radiolabel by 50{\%}; minimal detectable amounts of immunoreactive enzymes were 2 ng and 10 ng respectively. The assays were initially used to assess the effects of physiological perturbations (i.e. fasting and age) on enzyme concentrations in rat liver; these experiments showed that changes in enzyme concentrations do not always correlate with changes in enzyme activities. This represents the first report of radioimmunoassays for lysosomal glycosidases. The results suggest that these radioimmunoassays provide useful technology for the study of regulatory control mechanisms of the concentration of lysosomal glycosidases in mammalian tissues.",
author = "{De Groen}, {P. C.} and LeSage, {G. D.} and Tietz, {P. S.} and {La Russo}, {Nicholas F}",
year = "1989",
language = "English (US)",
volume = "264",
pages = "115--123",
journal = "Biochemical Journal",
issn = "0264-6021",
publisher = "Portland Press Ltd.",
number = "1",

}

TY - JOUR

T1 - Purification and immunological quantification of rat liver lysosomal glycosidases

AU - De Groen, P. C.

AU - LeSage, G. D.

AU - Tietz, P. S.

AU - La Russo, Nicholas F

PY - 1989

Y1 - 1989

N2 - Although lysosomal enzyme activities are known to vary in response to numerous physiological and pharmacological stimuli, the relationship between lysosomal enzyme activity and enzyme concentration has not been systematically studied. Therefore we developed radioimmunoassays for two lysosomal glycosidases in order to determine lysosomal enzyme concentration. β-Galactosidase and β-glucuronidase were purified from rat liver 2780-fold and 1280-fold respectively, by using differential centrifugation, affinity chromatography, ion-exchange chromatography and molecular-sieve chromatography. Polyclonal antibodies to these enzymes were raised in rabbits, and two radioimmunoassays were established. Antibody specificity was shown by: (i) selective immunoprecipitation of enzyme activity; (ii) identical bands of purified enzyme on SDS/polyacrylamide-gel electrophoresis and immunoelectrophoresis; (iii) single immunoreactive peaks in molecular-sieve chromatography experiments. Sensitivities of the assays were such that 15 ng of β-galactosidase and 45 ng of β-glucuronidase decreased the ratio of bound to free radiolabel by 50%; minimal detectable amounts of immunoreactive enzymes were 2 ng and 10 ng respectively. The assays were initially used to assess the effects of physiological perturbations (i.e. fasting and age) on enzyme concentrations in rat liver; these experiments showed that changes in enzyme concentrations do not always correlate with changes in enzyme activities. This represents the first report of radioimmunoassays for lysosomal glycosidases. The results suggest that these radioimmunoassays provide useful technology for the study of regulatory control mechanisms of the concentration of lysosomal glycosidases in mammalian tissues.

AB - Although lysosomal enzyme activities are known to vary in response to numerous physiological and pharmacological stimuli, the relationship between lysosomal enzyme activity and enzyme concentration has not been systematically studied. Therefore we developed radioimmunoassays for two lysosomal glycosidases in order to determine lysosomal enzyme concentration. β-Galactosidase and β-glucuronidase were purified from rat liver 2780-fold and 1280-fold respectively, by using differential centrifugation, affinity chromatography, ion-exchange chromatography and molecular-sieve chromatography. Polyclonal antibodies to these enzymes were raised in rabbits, and two radioimmunoassays were established. Antibody specificity was shown by: (i) selective immunoprecipitation of enzyme activity; (ii) identical bands of purified enzyme on SDS/polyacrylamide-gel electrophoresis and immunoelectrophoresis; (iii) single immunoreactive peaks in molecular-sieve chromatography experiments. Sensitivities of the assays were such that 15 ng of β-galactosidase and 45 ng of β-glucuronidase decreased the ratio of bound to free radiolabel by 50%; minimal detectable amounts of immunoreactive enzymes were 2 ng and 10 ng respectively. The assays were initially used to assess the effects of physiological perturbations (i.e. fasting and age) on enzyme concentrations in rat liver; these experiments showed that changes in enzyme concentrations do not always correlate with changes in enzyme activities. This represents the first report of radioimmunoassays for lysosomal glycosidases. The results suggest that these radioimmunoassays provide useful technology for the study of regulatory control mechanisms of the concentration of lysosomal glycosidases in mammalian tissues.

UR - http://www.scopus.com/inward/record.url?scp=0024454671&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024454671&partnerID=8YFLogxK

M3 - Article

VL - 264

SP - 115

EP - 123

JO - Biochemical Journal

JF - Biochemical Journal

SN - 0264-6021

IS - 1

ER -