PTEN/MMAC1 mutations and EGFR amplification in glioblastomas

Wanguo Liu, C. David James, Lori Frederick, Benjamin E. Alderete, Robert B. Jenkins

Research output: Contribution to journalArticle

172 Scopus citations

Abstract

Loss of heterozygosity (LOH) from chromosome 10 is a hallmark of glioblastoma, the most malignant (grade IV) form of glioma. A candidate tumor suppressor gene, PTEN/MMAC1, that may be targeted for deletion in association with chromosome 10 LOH has recently been identified. Here we have investigated 63 glioblastomas for PTEN/MMAC1 alterations and identified DNA sequence changes that would affect the encoded protein in 17 (275) tumors. Microsatellite analyses of normal-tumor DNA pairs were performed on 14 of these cases and revealed LOH at locations flanking and/or near PTEN/MMAC1 in all but 1 instance, suggesting that deletion of the remaining wild-type allele had occurred in the large majority of tumors with PTEN/MMAC1 mutations. Competitive PCR assays were developed to address the possible occurrence of PTEN/MMAC1 homozygous deletions in glioblastomas, and this analysis identified three samples having loss of both PTEN/MMAC1 alleles. EGFR amplification was determined to occur at similar frequencies among cases with or without PTEN/MMAC1 homozygous deletions or mutations, suggesting that a growth-promoting effect resulting from amplification-associated increases in epidermal growth factor receptor signaling is not necessarily dependent on the inactivation of PTEN/MMAC1.

Original languageEnglish (US)
Pages (from-to)5254-5257
Number of pages4
JournalCancer research
Volume57
Issue number23
StatePublished - Dec 1 1997

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Fingerprint Dive into the research topics of 'PTEN/MMAC1 mutations and EGFR amplification in glioblastomas'. Together they form a unique fingerprint.

  • Cite this

    Liu, W., James, C. D., Frederick, L., Alderete, B. E., & Jenkins, R. B. (1997). PTEN/MMAC1 mutations and EGFR amplification in glioblastomas. Cancer research, 57(23), 5254-5257.