TY - JOUR
T1 - Pseudogene INTS6P1 regulates its cognate gene INTS6 through competitive binding of miR-17-5p in hepatocellular carcinoma
AU - Peng, Haoran
AU - Ishida, Masaharu
AU - Li, Ling
AU - Saito, Atsushi
AU - Kamiya, Atsushi
AU - Hamilton, James P.
AU - Fu, Rongdang
AU - Olaru, Alexandru V.
AU - An, Fangmei
AU - Popescu, Irinel
AU - Iacob, Razvan
AU - Dima, Simona
AU - Alexandrescu, Sorin T.
AU - Grigorie, Razvan
AU - Nastase, Anca
AU - Berindan-Neagoe, Ioana
AU - Tomuleasa, Ciprian
AU - Graur, Florin
AU - Zaharia, Florin
AU - Torbenson, Michael S.
AU - Mezey, Esteban
AU - Lu, Minqiang
AU - Selaru, Florin M.
PY - 2015
Y1 - 2015
N2 - The complex regulation of tumor suppressive gene and its pseudogenes play key roles in the pathogenesis of hepatocellular cancer (HCC). However, the roles played by pseudogenes in the pathogenesis of HCC are still incompletely elucidated. This study identifies the putative tumor suppressor INTS6 and its pseudogene INTS6P1 in HCC through the whole genome microarray expression. Furthermore, the functional studies - include growth curves, cell death, migration assays and in vivo studies - verify the tumor suppressive roles of INTS6 and INTS6P1 in HCC. Finally, the mechanistic experiments indicate that INTS6 and INTS6P1 are reciprocally regulated through competition for oncomiR-17-5p. Taken together, these findings demonstrate INTS6P1 and INTS6 exert the tumor suppressive roles through competing for oncomiR-17-5p. Our investigation of this regulatory circuit reveals novel insights into the underlying mechanisms of hepatocarcinogenesis.
AB - The complex regulation of tumor suppressive gene and its pseudogenes play key roles in the pathogenesis of hepatocellular cancer (HCC). However, the roles played by pseudogenes in the pathogenesis of HCC are still incompletely elucidated. This study identifies the putative tumor suppressor INTS6 and its pseudogene INTS6P1 in HCC through the whole genome microarray expression. Furthermore, the functional studies - include growth curves, cell death, migration assays and in vivo studies - verify the tumor suppressive roles of INTS6 and INTS6P1 in HCC. Finally, the mechanistic experiments indicate that INTS6 and INTS6P1 are reciprocally regulated through competition for oncomiR-17-5p. Taken together, these findings demonstrate INTS6P1 and INTS6 exert the tumor suppressive roles through competing for oncomiR-17-5p. Our investigation of this regulatory circuit reveals novel insights into the underlying mechanisms of hepatocarcinogenesis.
KW - Competitive endogenous RNA
KW - Hepatocellular carcinoma
KW - INTS6
KW - Pseudogene
KW - Tumor suppressor
UR - http://www.scopus.com/inward/record.url?scp=84925645312&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84925645312&partnerID=8YFLogxK
U2 - 10.18632/oncotarget.3290
DO - 10.18632/oncotarget.3290
M3 - Article
C2 - 25686840
AN - SCOPUS:84925645312
SN - 1949-2553
VL - 6
SP - 5666
EP - 5677
JO - Oncotarget
JF - Oncotarget
IS - 8
ER -