PSD-95 mediates membrane clustering of the human plasma membrane Ca2+ pump isoform 4b

Rita Padányi, Katalin Pászty, Emanuel E. Strehler, Ágnes Enyedi

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Besides the control of global calcium changes, specific plasma membrane calcium ATPase (PMCA) isoforms are involved in the regulation of local calcium signals. Although local calcium signaling requires the confinement of signaling molecules into microdomains, little is known about the specific organization of PMCA molecules within the plasma membrane. Here we show that co-expression with the postsynaptic density-95 (PSD-95) scaffolding protein increased the plasma membrane expression of PMCA4b and redistributed the pump into clusters. The clustering of PMCA4b was fully dependent on the presence of its PDZ-binding sequence. Using the fluorescence recovery after photobleaching (FRAP) technique, we show that the lateral membrane mobility of the clustered PMCA4b is significantly lower than that of the non-clustered molecules. Disruption of the actin-based cytoskeleton by cytochalasin D resulted in increased cluster size. Our results suggest that PSD-95 promotes the formation of high-density PMCA4b microdomains in the plasma membrane and that the membrane cytoskeleton plays an important role in the regulation of this process.

Original languageEnglish (US)
Pages (from-to)1023-1032
Number of pages10
JournalBiochimica et Biophysica Acta - Molecular Cell Research
Volume1793
Issue number6
DOIs
StatePublished - Jun 2009

Fingerprint

Post-Synaptic Density
Plasma Membrane Calcium-Transporting ATPases
Cluster Analysis
Protein Isoforms
Cell Membrane
Membranes
Fluorescence Recovery After Photobleaching
Calcium
Cytochalasin D
Calcium Signaling
Cytoskeleton
Actin Cytoskeleton
Proteins

Keywords

  • Calcium pump
  • FRAP
  • Membrane clustering
  • PDZ protein
  • Plasma membrane Ca ATPase
  • PSD-95

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

PSD-95 mediates membrane clustering of the human plasma membrane Ca2+ pump isoform 4b. / Padányi, Rita; Pászty, Katalin; Strehler, Emanuel E.; Enyedi, Ágnes.

In: Biochimica et Biophysica Acta - Molecular Cell Research, Vol. 1793, No. 6, 06.2009, p. 1023-1032.

Research output: Contribution to journalArticle

Padányi, Rita ; Pászty, Katalin ; Strehler, Emanuel E. ; Enyedi, Ágnes. / PSD-95 mediates membrane clustering of the human plasma membrane Ca2+ pump isoform 4b. In: Biochimica et Biophysica Acta - Molecular Cell Research. 2009 ; Vol. 1793, No. 6. pp. 1023-1032.
@article{cf1198a5a1d444099ce448888df0ef5e,
title = "PSD-95 mediates membrane clustering of the human plasma membrane Ca2+ pump isoform 4b",
abstract = "Besides the control of global calcium changes, specific plasma membrane calcium ATPase (PMCA) isoforms are involved in the regulation of local calcium signals. Although local calcium signaling requires the confinement of signaling molecules into microdomains, little is known about the specific organization of PMCA molecules within the plasma membrane. Here we show that co-expression with the postsynaptic density-95 (PSD-95) scaffolding protein increased the plasma membrane expression of PMCA4b and redistributed the pump into clusters. The clustering of PMCA4b was fully dependent on the presence of its PDZ-binding sequence. Using the fluorescence recovery after photobleaching (FRAP) technique, we show that the lateral membrane mobility of the clustered PMCA4b is significantly lower than that of the non-clustered molecules. Disruption of the actin-based cytoskeleton by cytochalasin D resulted in increased cluster size. Our results suggest that PSD-95 promotes the formation of high-density PMCA4b microdomains in the plasma membrane and that the membrane cytoskeleton plays an important role in the regulation of this process.",
keywords = "Calcium pump, FRAP, Membrane clustering, PDZ protein, Plasma membrane Ca ATPase, PSD-95",
author = "Rita Pad{\'a}nyi and Katalin P{\'a}szty and Strehler, {Emanuel E.} and {\'A}gnes Enyedi",
year = "2009",
month = "6",
doi = "10.1016/j.bbamcr.2008.11.007",
language = "English (US)",
volume = "1793",
pages = "1023--1032",
journal = "Biochimica et Biophysica Acta - Molecular Cell Research",
issn = "0167-4889",
publisher = "Elsevier",
number = "6",

}

TY - JOUR

T1 - PSD-95 mediates membrane clustering of the human plasma membrane Ca2+ pump isoform 4b

AU - Padányi, Rita

AU - Pászty, Katalin

AU - Strehler, Emanuel E.

AU - Enyedi, Ágnes

PY - 2009/6

Y1 - 2009/6

N2 - Besides the control of global calcium changes, specific plasma membrane calcium ATPase (PMCA) isoforms are involved in the regulation of local calcium signals. Although local calcium signaling requires the confinement of signaling molecules into microdomains, little is known about the specific organization of PMCA molecules within the plasma membrane. Here we show that co-expression with the postsynaptic density-95 (PSD-95) scaffolding protein increased the plasma membrane expression of PMCA4b and redistributed the pump into clusters. The clustering of PMCA4b was fully dependent on the presence of its PDZ-binding sequence. Using the fluorescence recovery after photobleaching (FRAP) technique, we show that the lateral membrane mobility of the clustered PMCA4b is significantly lower than that of the non-clustered molecules. Disruption of the actin-based cytoskeleton by cytochalasin D resulted in increased cluster size. Our results suggest that PSD-95 promotes the formation of high-density PMCA4b microdomains in the plasma membrane and that the membrane cytoskeleton plays an important role in the regulation of this process.

AB - Besides the control of global calcium changes, specific plasma membrane calcium ATPase (PMCA) isoforms are involved in the regulation of local calcium signals. Although local calcium signaling requires the confinement of signaling molecules into microdomains, little is known about the specific organization of PMCA molecules within the plasma membrane. Here we show that co-expression with the postsynaptic density-95 (PSD-95) scaffolding protein increased the plasma membrane expression of PMCA4b and redistributed the pump into clusters. The clustering of PMCA4b was fully dependent on the presence of its PDZ-binding sequence. Using the fluorescence recovery after photobleaching (FRAP) technique, we show that the lateral membrane mobility of the clustered PMCA4b is significantly lower than that of the non-clustered molecules. Disruption of the actin-based cytoskeleton by cytochalasin D resulted in increased cluster size. Our results suggest that PSD-95 promotes the formation of high-density PMCA4b microdomains in the plasma membrane and that the membrane cytoskeleton plays an important role in the regulation of this process.

KW - Calcium pump

KW - FRAP

KW - Membrane clustering

KW - PDZ protein

KW - Plasma membrane Ca ATPase

KW - PSD-95

UR - http://www.scopus.com/inward/record.url?scp=67349288065&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=67349288065&partnerID=8YFLogxK

U2 - 10.1016/j.bbamcr.2008.11.007

DO - 10.1016/j.bbamcr.2008.11.007

M3 - Article

C2 - 19073225

AN - SCOPUS:67349288065

VL - 1793

SP - 1023

EP - 1032

JO - Biochimica et Biophysica Acta - Molecular Cell Research

JF - Biochimica et Biophysica Acta - Molecular Cell Research

SN - 0167-4889

IS - 6

ER -