Protocol for M3P: A comprehensive and clinical oriented targeted sequencing panel for routine molecular analysis in multiple myeloma

Santiago Barrio, Matteo DáVia, Laura Bruins, Thorsten Stühmer, Torsten Steinbrunn, Max Bittrich, Hermann Einsele, Alexander Keith Stewart, Esteban Braggio, Klaus Martin Kortüm

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Scopus citations

Abstract

Over the past 10 years next generation sequencing (NGS) approaches deciphered a large number of genomes from a wide variety of tumor types. However, despite most relevant findings, this technology has not yet been implemented into standard diagnostic workflows. Broad access to NGS technology is still limited, sequencing/analysis times exceed clinically relevant timeframes and despite huge cuts, costs remain significant. We proposed a custom-tailored gene panel, which focuses on a selected number of relevant genes and developed a clinically oriented NGS targeted sequencing approach for the molecular characterization of Multiple Myeloma (MM) tumors, allowing the description of the tumor genetic heterogeneity and its changes under selective pressure of antitumor therapy, in a more cost effective and faster turnaround timeframe.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages117-128
Number of pages12
DOIs
StatePublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1792
ISSN (Print)1064-3745

Keywords

  • Multiple myeloma
  • Next generation sequencing
  • Standard diagnostic workflow
  • Targeted sequencing panel

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Fingerprint Dive into the research topics of 'Protocol for M<sup>3</sup>P: A comprehensive and clinical oriented targeted sequencing panel for routine molecular analysis in multiple myeloma'. Together they form a unique fingerprint.

  • Cite this

    Barrio, S., DáVia, M., Bruins, L., Stühmer, T., Steinbrunn, T., Bittrich, M., Einsele, H., Stewart, A. K., Braggio, E., & Kortüm, K. M. (2018). Protocol for M3P: A comprehensive and clinical oriented targeted sequencing panel for routine molecular analysis in multiple myeloma. In Methods in Molecular Biology (pp. 117-128). (Methods in Molecular Biology; Vol. 1792). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7865-6_8