Protocol for M3P: A comprehensive and clinical oriented targeted sequencing panel for routine molecular analysis in multiple myeloma

Santiago Barrio; Matteo DáVia; Laura Bruins; Thorsten Stühmer; Torsten Steinbrunn; Max Bittrich; Hermann Einsele; Alexander Keith Stewart; Esteban Braggio; Klaus Martin Kortüm

doi:10.1007/978-1-4939-7865-6_8

Protocol for M³P: A comprehensive and clinical oriented targeted sequencing panel for routine molecular analysis in multiple myeloma

Santiago Barrio, Matteo DáVia, Laura Bruins, Thorsten Stühmer, Torsten Steinbrunn, Max Bittrich, Hermann Einsele, Alexander Keith Stewart, Esteban Braggio, Klaus Martin Kortüm

Research output: Chapter in Book/Report/Conference proceeding › Chapter

3 Scopus citations

Abstract

Over the past 10 years next generation sequencing (NGS) approaches deciphered a large number of genomes from a wide variety of tumor types. However, despite most relevant findings, this technology has not yet been implemented into standard diagnostic workflows. Broad access to NGS technology is still limited, sequencing/analysis times exceed clinically relevant timeframes and despite huge cuts, costs remain significant. We proposed a custom-tailored gene panel, which focuses on a selected number of relevant genes and developed a clinically oriented NGS targeted sequencing approach for the molecular characterization of Multiple Myeloma (MM) tumors, allowing the description of the tumor genetic heterogeneity and its changes under selective pressure of antitumor therapy, in a more cost effective and faster turnaround timeframe.

Original language	English (US)
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	117-128
Number of pages	12
DOIs	https://doi.org/10.1007/978-1-4939-7865-6_8
State	Published - 2018

Publication series

Name	Methods in Molecular Biology
Volume	1792
ISSN (Print)	1064-3745

Keywords

Multiple myeloma
Next generation sequencing
Standard diagnostic workflow
Targeted sequencing panel

ASJC Scopus subject areas

Molecular Biology
Genetics

Access to Document

10.1007/978-1-4939-7865-6_8

Cite this

Barrio, S., DáVia, M., Bruins, L., Stühmer, T., Steinbrunn, T., Bittrich, M., Einsele, H., Stewart, A. K., Braggio, E., & Kortüm, K. M. (2018). Protocol for M³P: A comprehensive and clinical oriented targeted sequencing panel for routine molecular analysis in multiple myeloma. In Methods in Molecular Biology (pp. 117-128). (Methods in Molecular Biology; Vol. 1792). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7865-6_8

Protocol for M³P: A comprehensive and clinical oriented targeted sequencing panel for routine molecular analysis in multiple myeloma. / Barrio, Santiago; DáVia, Matteo; Bruins, Laura et al.
Methods in Molecular Biology. Humana Press Inc., 2018. p. 117-128 (Methods in Molecular Biology; Vol. 1792).

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Barrio, S, DáVia, M, Bruins, L, Stühmer, T, Steinbrunn, T, Bittrich, M, Einsele, H, Stewart, AK, Braggio, E & Kortüm, KM 2018, Protocol for M³P: A comprehensive and clinical oriented targeted sequencing panel for routine molecular analysis in multiple myeloma. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1792, Humana Press Inc., pp. 117-128. https://doi.org/10.1007/978-1-4939-7865-6_8

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