Proteomics of human aqueous humor

Krishna R. Murthy, Pavithra Rajagopalan, Sneha M. Pinto, Jayshree Advani, Praveen R. Murthy, Renu Goel, Yashwanth Subbannayya, Lavanya Balakrishnan, Mahashweta Dash, Abhijith K. Anil, Srikanth S. Manda, Raja Sekhar Nirujogi, Dhanashree S. Kelkar, Gajanan J. Sathe, Gourav Dey, Aditi Chatterjee, Harsha Gowda, Shukti Chakravarti, Subramanian Shankar, Nandini A. SahasrabuddheBipin Nair, Babu Lal Somani, T. S.Keshava Prasad, Akhilesh Pandey

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The aqueous humor is a colorless, transparent fluid that fills the anterior chamber of the eye. It plays an important role in maintaining the intraocular pressure and providing nourishment to the lens and cornea. The constitution of the aqueous humor is controlled by the blood-aqueous barrier. Though this ocular fluid has been extensively studied, its role in ocular physiology is still not completely understood. In this study, aqueous humor samples were collected from 250 patients undergoing cataract surgery, subjected to multiple fractionation strategies and analyzed on a Fourier transform LTQ-Orbitrap Velos mass spectrometer. In all, we identified 763 proteins, of which 386 have been identified for the first time in this study. Sorbitol dehydrogenase (SORD), filensin (BFSP1), and phakinin (BFSP2) are some of the proteins that have not been previously reported in the aqueous humor. Gene Ontology analysis revealed 35% of the identified proteins to be extracellular, with a majority of them involved in cell communication and signal transduction. This study comprehensively reports 386 novel proteins that have important potential as biomarker candidates for future research into personalized medicine and diagnostics aimed towards improving visual health.

Original languageEnglish (US)
Pages (from-to)283-293
Number of pages11
JournalOMICS A Journal of Integrative Biology
Volume19
Issue number5
DOIs
StatePublished - May 1 2015
Externally publishedYes

Fingerprint

Aqueous Humor
Proteomics
Proteins
Ocular Physiological Phenomena
Blood-Aqueous Barrier
L-Iditol 2-Dehydrogenase
Signal transduction
Precision Medicine
Gene Ontology
Fluids
Constitution and Bylaws
Physiology
Anterior Chamber
Biomarkers
Mass spectrometers
Fourier Analysis
Fractionation
Intraocular Pressure
Cell Communication
Cornea

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Genetics

Cite this

Murthy, K. R., Rajagopalan, P., Pinto, S. M., Advani, J., Murthy, P. R., Goel, R., ... Pandey, A. (2015). Proteomics of human aqueous humor. OMICS A Journal of Integrative Biology, 19(5), 283-293. https://doi.org/10.1089/omi.2015.0029

Proteomics of human aqueous humor. / Murthy, Krishna R.; Rajagopalan, Pavithra; Pinto, Sneha M.; Advani, Jayshree; Murthy, Praveen R.; Goel, Renu; Subbannayya, Yashwanth; Balakrishnan, Lavanya; Dash, Mahashweta; Anil, Abhijith K.; Manda, Srikanth S.; Nirujogi, Raja Sekhar; Kelkar, Dhanashree S.; Sathe, Gajanan J.; Dey, Gourav; Chatterjee, Aditi; Gowda, Harsha; Chakravarti, Shukti; Shankar, Subramanian; Sahasrabuddhe, Nandini A.; Nair, Bipin; Somani, Babu Lal; Prasad, T. S.Keshava; Pandey, Akhilesh.

In: OMICS A Journal of Integrative Biology, Vol. 19, No. 5, 01.05.2015, p. 283-293.

Research output: Contribution to journalArticle

Murthy, KR, Rajagopalan, P, Pinto, SM, Advani, J, Murthy, PR, Goel, R, Subbannayya, Y, Balakrishnan, L, Dash, M, Anil, AK, Manda, SS, Nirujogi, RS, Kelkar, DS, Sathe, GJ, Dey, G, Chatterjee, A, Gowda, H, Chakravarti, S, Shankar, S, Sahasrabuddhe, NA, Nair, B, Somani, BL, Prasad, TSK & Pandey, A 2015, 'Proteomics of human aqueous humor', OMICS A Journal of Integrative Biology, vol. 19, no. 5, pp. 283-293. https://doi.org/10.1089/omi.2015.0029
Murthy KR, Rajagopalan P, Pinto SM, Advani J, Murthy PR, Goel R et al. Proteomics of human aqueous humor. OMICS A Journal of Integrative Biology. 2015 May 1;19(5):283-293. https://doi.org/10.1089/omi.2015.0029
Murthy, Krishna R. ; Rajagopalan, Pavithra ; Pinto, Sneha M. ; Advani, Jayshree ; Murthy, Praveen R. ; Goel, Renu ; Subbannayya, Yashwanth ; Balakrishnan, Lavanya ; Dash, Mahashweta ; Anil, Abhijith K. ; Manda, Srikanth S. ; Nirujogi, Raja Sekhar ; Kelkar, Dhanashree S. ; Sathe, Gajanan J. ; Dey, Gourav ; Chatterjee, Aditi ; Gowda, Harsha ; Chakravarti, Shukti ; Shankar, Subramanian ; Sahasrabuddhe, Nandini A. ; Nair, Bipin ; Somani, Babu Lal ; Prasad, T. S.Keshava ; Pandey, Akhilesh. / Proteomics of human aqueous humor. In: OMICS A Journal of Integrative Biology. 2015 ; Vol. 19, No. 5. pp. 283-293.
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