Proteomics for understanding miRNA biology

Tai Chung Huang, Sneha M. Pinto, Akhilesh Pandey

Research output: Contribution to journalReview article

19 Scopus citations

Abstract

MicroRNAs (miRNAs) are small noncoding RNAs that play important roles in posttranscriptional regulation of gene expression. Mature miRNAs associate with the RNA interference silencing complex to repress mRNA translation and/or degrade mRNA transcripts. Mass spectrometry-based proteomics has enabled identification of several core components of the canonical miRNA processing pathway and their posttranslational modifications which are pivotal in miRNA regulatory mechanisms. The use of quantitative proteomic strategies has also emerged as a key technique for experimental identification of miRNA targets by allowing direct determination of proteins whose levels are altered because of translational suppression. This review focuses on the role of proteomics and labeling strategies to understand miRNA biology.

Original languageEnglish (US)
Pages (from-to)558-567
Number of pages10
JournalProteomics
Volume13
Issue number3-4
DOIs
StatePublished - Feb 1 2013

Keywords

  • Cell biology
  • ITRAQ
  • MiRNA
  • Multiple reaction monitoring
  • Noncoding RNA
  • SILAC

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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