Proteomics for understanding miRNA biology

Tai Chung Huang; Sneha M. Pinto; Akhilesh Pandey

doi:10.1002/pmic.201200339

Proteomics for understanding miRNA biology

Tai Chung Huang, Sneha M. Pinto, Akhilesh Pandey

Laboratory Medicine and Pathology

Research output: Contribution to journal › Review article › peer-review

21 Scopus citations

Abstract

MicroRNAs (miRNAs) are small noncoding RNAs that play important roles in posttranscriptional regulation of gene expression. Mature miRNAs associate with the RNA interference silencing complex to repress mRNA translation and/or degrade mRNA transcripts. Mass spectrometry-based proteomics has enabled identification of several core components of the canonical miRNA processing pathway and their posttranslational modifications which are pivotal in miRNA regulatory mechanisms. The use of quantitative proteomic strategies has also emerged as a key technique for experimental identification of miRNA targets by allowing direct determination of proteins whose levels are altered because of translational suppression. This review focuses on the role of proteomics and labeling strategies to understand miRNA biology.

Original language	English (US)
Pages (from-to)	558-567
Number of pages	10
Journal	Proteomics
Volume	13
Issue number	3-4
DOIs	https://doi.org/10.1002/pmic.201200339
State	Published - Feb 2013

Keywords

Cell biology
ITRAQ
MiRNA
Multiple reaction monitoring
Noncoding RNA
SILAC

ASJC Scopus subject areas

Biochemistry
Molecular Biology

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10.1002/pmic.201200339

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title = "Proteomics for understanding miRNA biology",

abstract = "MicroRNAs (miRNAs) are small noncoding RNAs that play important roles in posttranscriptional regulation of gene expression. Mature miRNAs associate with the RNA interference silencing complex to repress mRNA translation and/or degrade mRNA transcripts. Mass spectrometry-based proteomics has enabled identification of several core components of the canonical miRNA processing pathway and their posttranslational modifications which are pivotal in miRNA regulatory mechanisms. The use of quantitative proteomic strategies has also emerged as a key technique for experimental identification of miRNA targets by allowing direct determination of proteins whose levels are altered because of translational suppression. This review focuses on the role of proteomics and labeling strategies to understand miRNA biology.",

keywords = "Cell biology, ITRAQ, MiRNA, Multiple reaction monitoring, Noncoding RNA, SILAC",

author = "Huang, {Tai Chung} and Pinto, {Sneha M.} and Akhilesh Pandey",

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AU - Huang, Tai Chung

AU - Pinto, Sneha M.

AU - Pandey, Akhilesh

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AB - MicroRNAs (miRNAs) are small noncoding RNAs that play important roles in posttranscriptional regulation of gene expression. Mature miRNAs associate with the RNA interference silencing complex to repress mRNA translation and/or degrade mRNA transcripts. Mass spectrometry-based proteomics has enabled identification of several core components of the canonical miRNA processing pathway and their posttranslational modifications which are pivotal in miRNA regulatory mechanisms. The use of quantitative proteomic strategies has also emerged as a key technique for experimental identification of miRNA targets by allowing direct determination of proteins whose levels are altered because of translational suppression. This review focuses on the role of proteomics and labeling strategies to understand miRNA biology.

KW - Cell biology

KW - ITRAQ

KW - MiRNA

KW - Multiple reaction monitoring

KW - Noncoding RNA

KW - SILAC

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JO - Proteomics

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Proteomics for understanding miRNA biology

Abstract

Keywords

ASJC Scopus subject areas

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