Proteomics-based identification of proteins with altered expression induced by 12-O-tetradecanoylphorbol 13-acetate in nasopharyngeal carcinoma CNE2 cells

Peizhou Jiang, Ming Gan, Hua Huang, Xin Ming Shen, Shuang Wang, Kai Tai Yao

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Nasopharyngeal carcinoma (NPC) is a malignancy with high incidence in Southern China and South-East Asia. Etiology studies indicate that chemical carcinogen promoters, such as 12-O-tetradecanoylphorbol-13-acetate (TPA), are important factors causing NPC development. However, the mechanism of the TPA effect on NPC remains unclear. In the present study, cells from a poorly differentiated squamous cell carcinoma NPC cell line, CNE2, were stimulated by TPA and proteomics technology was carried out to find protein discrepancies between control and TPA-treated cells. Results revealed that TPA treatment in CNE2 cells could upregulate the expression of "triosephosphate isomerase" and "14-3-3 protein sigma" and downregulate the expression of "reticulocalbin 1 precursor", "nucleophosmin", "mitochondrial matrix protein p1 precursor", and "stathmin" . The changes in the expression of these genes suggested that TPA induced CNE2 cells to antiproliferation and to apoptosis, which was confirmed by subsequent apoptosis detection. Therefore, the effects of TPA on nasopharyngeal carcinoma cells were distinct from the effects on primary epithelial cells and we suggest reasons for these differences.

Original languageEnglish (US)
Pages (from-to)97-106
Number of pages10
JournalActa Biochimica et Biophysica Sinica
Volume37
Issue number2
StatePublished - Feb 1 2005
Externally publishedYes

Fingerprint

Tetradecanoylphorbol Acetate
Proteomics
Acetates
Proteins
Cells
Stathmin
Triose-Phosphate Isomerase
Apoptosis
14-3-3 Proteins
Protein Precursors
Far East
Mitochondrial Proteins
Nasopharyngeal carcinoma
Carcinogens
Squamous Cell Carcinoma
China
Up-Regulation
Down-Regulation
Genes
Epithelial Cells

Keywords

  • 12-O-tetradecanoyl-phorbol-13-acetate
  • Apoptosis
  • Nasopharyngeal carcinoma
  • Proteomics

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry

Cite this

Proteomics-based identification of proteins with altered expression induced by 12-O-tetradecanoylphorbol 13-acetate in nasopharyngeal carcinoma CNE2 cells. / Jiang, Peizhou; Gan, Ming; Huang, Hua; Shen, Xin Ming; Wang, Shuang; Yao, Kai Tai.

In: Acta Biochimica et Biophysica Sinica, Vol. 37, No. 2, 01.02.2005, p. 97-106.

Research output: Contribution to journalArticle

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