Proteomic approach for identification of serine/threonine phosphorylated proteins

Mads Grønborg, Troels Z. Kristiansen, Allan Stensballe, Jens S. Andersen, Matthias Mann, Akhilesh Pandey, Ole N. Jensen

Research output: Contribution to conferencePaperpeer-review

Abstract

Identification of serine/threonine phosphorylated proteins was carried out using proteomic approach. Various antibodies that recognized phosphoserine/phosphothreonine containing proteins were characterized by Western blotting. A differential display proteomics study using Calyculin A treated or non-treated HeLa cells and immunoprecipitation proteins with antipSer antibody was applied. The proteomic approach resulted in identification of several knowm as well as novel phosphoproteins.

Original languageEnglish (US)
Pages309-310
Number of pages2
StatePublished - Dec 1 2002
EventProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: Jun 2 2002Jun 6 2002

Other

OtherProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
CountryUnited States
CityOrlando, FL
Period6/2/026/6/02

ASJC Scopus subject areas

  • Spectroscopy

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