Proteomic approach for identification of serine/threonine phosphorylated proteins

Mads Grønborg, Troels Z. Kristiansen, Allan Stensballe, Jens S. Andersen, Matthias Mann, Akhilesh Pandey, Ole N. Jensen

Research output: Contribution to conferencePaper

Abstract

Identification of serine/threonine phosphorylated proteins was carried out using proteomic approach. Various antibodies that recognized phosphoserine/phosphothreonine containing proteins were characterized by Western blotting. A differential display proteomics study using Calyculin A treated or non-treated HeLa cells and immunoprecipitation proteins with antipSer antibody was applied. The proteomic approach resulted in identification of several knowm as well as novel phosphoproteins.

Original languageEnglish (US)
Pages309-310
Number of pages2
StatePublished - Dec 1 2002
Externally publishedYes
EventProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: Jun 2 2002Jun 6 2002

Other

OtherProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
CountryUnited States
CityOrlando, FL
Period6/2/026/6/02

Fingerprint

Threonine
Serine
Phosphothreonine
Phosphoserine
Proteins
Antibodies
Phosphoproteins
Display devices
Proteomics

ASJC Scopus subject areas

  • Spectroscopy

Cite this

Grønborg, M., Kristiansen, T. Z., Stensballe, A., Andersen, J. S., Mann, M., Pandey, A., & Jensen, O. N. (2002). Proteomic approach for identification of serine/threonine phosphorylated proteins. 309-310. Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States.

Proteomic approach for identification of serine/threonine phosphorylated proteins. / Grønborg, Mads; Kristiansen, Troels Z.; Stensballe, Allan; Andersen, Jens S.; Mann, Matthias; Pandey, Akhilesh; Jensen, Ole N.

2002. 309-310 Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States.

Research output: Contribution to conferencePaper

Grønborg, M, Kristiansen, TZ, Stensballe, A, Andersen, JS, Mann, M, Pandey, A & Jensen, ON 2002, 'Proteomic approach for identification of serine/threonine phosphorylated proteins', Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States, 6/2/02 - 6/6/02 pp. 309-310.
Grønborg M, Kristiansen TZ, Stensballe A, Andersen JS, Mann M, Pandey A et al. Proteomic approach for identification of serine/threonine phosphorylated proteins. 2002. Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States.
Grønborg, Mads ; Kristiansen, Troels Z. ; Stensballe, Allan ; Andersen, Jens S. ; Mann, Matthias ; Pandey, Akhilesh ; Jensen, Ole N. / Proteomic approach for identification of serine/threonine phosphorylated proteins. Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States.2 p.
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