Proteomic analysis of vascular endothelial cells-effects of laminar shear stress and high glucose

Xiao Li Wang, Alex Fu, Craig Spiro, Hon Chi Lee

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

This study directly measured the relative protein levels in bovine aortic endothelial cells (BAEC) that were cultured for two weeks in normal (5 mM, NG) or high (22 mM, HG) glucose and then were subjected to laminar shear stress at 0 or 15 dynes/cm2. Membrane preparations were labeled with one of the four isobaric tagging reagents (iTRAQ), followed by LC-MS/MS analysis. The results showed that HG and/or shear stress induced alterations in various membrane associated proteins involving many signaling pathways. While shear stress induced an increase in heat shock proteins and protein ubiquitination, which remained enhanced in HG, the effects of shear stress on the mechanosensing and protein phosphorylation pathways were altered by HG. These results were validated by Western blot analysis, suggesting that HG importantly modulates shear stress-mediated endothelial function.

Original languageEnglish (US)
Pages (from-to)445-454
Number of pages10
JournalJournal of Proteomics and Bioinformatics
Volume2
Issue number10
DOIs
StatePublished - Oct 2009

Fingerprint

Endothelial cells
Proteomics
Glucose
Shear stress
Endothelial Cells
Proteins
Ubiquitination
Heat-Shock Proteins
Membrane Proteins
Membranes
Western Blotting
Phosphorylation

Keywords

  • Diabetes
  • Endothelial cells
  • Glucose
  • Hyperglycemia
  • ITRAQ
  • MS/MS
  • Proteomic profiling
  • Shear stress

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology
  • Computer Science Applications

Cite this

Proteomic analysis of vascular endothelial cells-effects of laminar shear stress and high glucose. / Wang, Xiao Li; Fu, Alex; Spiro, Craig; Lee, Hon Chi.

In: Journal of Proteomics and Bioinformatics, Vol. 2, No. 10, 10.2009, p. 445-454.

Research output: Contribution to journalArticle

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