Proteomic analysis of the abomasal mucosal response following infection by the nematode, Haemonchus contortus, in genetically resistant and susceptible sheep

Shivashankar H. Nagaraj, H. C. Harsha, Antonio Reverter, Michelle L. Colgrave, Rakesh Sharma, Nicholas Andronicos, Peter Hunt, Moira Menzies, Michael S. Lees, Nirujogi R. Sekhar, Akhilesh Pandey, Aaron Ingham

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Sheep have a variable ability to resist gastrointestinal nematode infection, but the key factors mediating this response are poorly defined. Here we report the first large-scale application of quantitative proteomic technologies to define proteins that are differentially abundant between sheep selectively bred to have an enhanced (resistant) or reduced (susceptible) ability to eliminate nematodes. Samples were collected from the abomasal mucosa three days after experimental challenge with the nematode, Haemonchus contortus. This timing reflects the initial interaction of host and parasite, and the tissue represents the immediate interface. We identified and quantified more than 4400 unique proteins, of which 158 proteins showed > 1.5 fold difference between the resistant and susceptible sheep. Trefoil factor 2, a member of RAS oncogene family (RAP1A) and ring finger protein 126 were amongst the proteins found to be highly abundant in the abomasal surface of resistant sheep, whereas adenosine deaminase and the gastrokine-3 like precursor were found at higher levels in susceptible sheep. Construction of gut proteome interaction networks identified mitochondrial function and energetic partitioning as important components of an effective nematode eliminating response. The differentially abundant proteins may be useful targets for phenotypic tests that aim to identify sheep with an enhanced ability to resist nematode infection.

Original languageEnglish (US)
Pages (from-to)2141-2152
Number of pages12
JournalJournal of Proteomics
Volume75
Issue number7
DOIs
StatePublished - Apr 3 2012
Externally publishedYes

Fingerprint

Nematode Infections
Haemonchus
Proteomics
Sheep
Proteins
Host-Parasite Interactions
Adenosine Deaminase
Proteome
Oncogenes
Fingers
Mucous Membrane
Tissue
Technology

Keywords

  • Biological networks
  • Host-parasite
  • ITRAQ
  • Nematode resistant
  • Proteomic profiling
  • Sheep

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry

Cite this

Proteomic analysis of the abomasal mucosal response following infection by the nematode, Haemonchus contortus, in genetically resistant and susceptible sheep. / Nagaraj, Shivashankar H.; Harsha, H. C.; Reverter, Antonio; Colgrave, Michelle L.; Sharma, Rakesh; Andronicos, Nicholas; Hunt, Peter; Menzies, Moira; Lees, Michael S.; Sekhar, Nirujogi R.; Pandey, Akhilesh; Ingham, Aaron.

In: Journal of Proteomics, Vol. 75, No. 7, 03.04.2012, p. 2141-2152.

Research output: Contribution to journalArticle

Nagaraj, SH, Harsha, HC, Reverter, A, Colgrave, ML, Sharma, R, Andronicos, N, Hunt, P, Menzies, M, Lees, MS, Sekhar, NR, Pandey, A & Ingham, A 2012, 'Proteomic analysis of the abomasal mucosal response following infection by the nematode, Haemonchus contortus, in genetically resistant and susceptible sheep', Journal of Proteomics, vol. 75, no. 7, pp. 2141-2152. https://doi.org/10.1016/j.jprot.2012.01.016
Nagaraj, Shivashankar H. ; Harsha, H. C. ; Reverter, Antonio ; Colgrave, Michelle L. ; Sharma, Rakesh ; Andronicos, Nicholas ; Hunt, Peter ; Menzies, Moira ; Lees, Michael S. ; Sekhar, Nirujogi R. ; Pandey, Akhilesh ; Ingham, Aaron. / Proteomic analysis of the abomasal mucosal response following infection by the nematode, Haemonchus contortus, in genetically resistant and susceptible sheep. In: Journal of Proteomics. 2012 ; Vol. 75, No. 7. pp. 2141-2152.
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