Proteomic analysis of Streptomyces sp. ssr-198 grown on paddy straw

Surender Singh, Rameshwar Tiwari, Santosh Renuse, Kumar Pranaw, Lata Nain

Research output: Contribution to journalArticlepeer-review

10 Scopus citations


The filamentous bacteria Streptomyces spp. produces diverse extracellular enzymes and other secondary metabolites. Proteomic analysis of the secretome of holocellulolytic Streptomyces sp. ssr-198 was done by tandem mass spectrometry using an Orbitrap Velos hybrid mass spectrometer. A wide range of hydrolytic enzymes, including glycoside hydrolases (17), proteases (17), polysaccharide lyases (3), esterases (2), and hypothetical proteins (14) were detected in the secretome analyzed. Overall, the secretome composition constituted of 12.50% cellulases, 17.50% hemicellulases, 21.25% proteases, 17.50% hypothetical proteins, and 31.25% other proteins. Comprehensive analysis of secretome will be useful in gaining better understanding of the unique role of hydrolytic enzymes in lignocellulose hydrolysis and helps in determining the industrial applications of these potent enzymes.

Original languageEnglish (US)
Pages (from-to)790-797
Number of pages8
JournalJournal of Basic Microbiology
Issue number6
StatePublished - Jun 1 2015


  • Bioethanol
  • Holocellulase
  • Orbitrap
  • Paddy straw
  • Streptomyces

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology


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