Proteomic Analysis of Plasmodium Merosomes: The Link between Liver and Blood Stages in Malaria

Melanie J. Shears, Raja Sekhar Nirujogi, Kristian E. Swearingen, Santosh Renuse, Satish Mishra, Panga Jaipal Reddy, Robert L. Moritz, Akhilesh Pandey, Photini Sinnis

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The pre-erythrocytic liver stage of the malaria parasite, comprising sporozoites and the liver stages into which they develop, remains one of the least understood parts of the lifecycle, in part owing to the low numbers of parasites. Nonetheless, it is recognized as an important target for antimalarial drugs and vaccines. Here we provide the first proteomic analysis of merosomes, which define the final phase of the liver stage and are responsible for initiating the blood stage of infection. We identify a total of 1879 parasite proteins, and a core set of 1188 proteins quantitatively detected in every biological replicate, providing an extensive picture of the protein repertoire of this stage. This unique data set will allow us to explore key questions about the biology of merosomes and hepatic merozoites.

Original languageEnglish (US)
Pages (from-to)3404-3418
Number of pages15
JournalJournal of Proteome Research
Volume18
Issue number9
DOIs
StatePublished - Sep 6 2019

Fingerprint

Plasmodium
Liver
Proteomics
Malaria
Blood
Parasites
Proteins
Antimalarials
Merozoites
Sporozoites
Vaccines
Infection

Keywords

  • exoerythrocytic stage
  • liver stages
  • malaria
  • merosomes
  • PEXEL
  • proteomics
  • sporozoites

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

Cite this

Shears, M. J., Sekhar Nirujogi, R., Swearingen, K. E., Renuse, S., Mishra, S., Jaipal Reddy, P., ... Sinnis, P. (2019). Proteomic Analysis of Plasmodium Merosomes: The Link between Liver and Blood Stages in Malaria. Journal of Proteome Research, 18(9), 3404-3418. https://doi.org/10.1021/acs.jproteome.9b00324

Proteomic Analysis of Plasmodium Merosomes : The Link between Liver and Blood Stages in Malaria. / Shears, Melanie J.; Sekhar Nirujogi, Raja; Swearingen, Kristian E.; Renuse, Santosh; Mishra, Satish; Jaipal Reddy, Panga; Moritz, Robert L.; Pandey, Akhilesh; Sinnis, Photini.

In: Journal of Proteome Research, Vol. 18, No. 9, 06.09.2019, p. 3404-3418.

Research output: Contribution to journalArticle

Shears, MJ, Sekhar Nirujogi, R, Swearingen, KE, Renuse, S, Mishra, S, Jaipal Reddy, P, Moritz, RL, Pandey, A & Sinnis, P 2019, 'Proteomic Analysis of Plasmodium Merosomes: The Link between Liver and Blood Stages in Malaria', Journal of Proteome Research, vol. 18, no. 9, pp. 3404-3418. https://doi.org/10.1021/acs.jproteome.9b00324
Shears MJ, Sekhar Nirujogi R, Swearingen KE, Renuse S, Mishra S, Jaipal Reddy P et al. Proteomic Analysis of Plasmodium Merosomes: The Link between Liver and Blood Stages in Malaria. Journal of Proteome Research. 2019 Sep 6;18(9):3404-3418. https://doi.org/10.1021/acs.jproteome.9b00324
Shears, Melanie J. ; Sekhar Nirujogi, Raja ; Swearingen, Kristian E. ; Renuse, Santosh ; Mishra, Satish ; Jaipal Reddy, Panga ; Moritz, Robert L. ; Pandey, Akhilesh ; Sinnis, Photini. / Proteomic Analysis of Plasmodium Merosomes : The Link between Liver and Blood Stages in Malaria. In: Journal of Proteome Research. 2019 ; Vol. 18, No. 9. pp. 3404-3418.
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