Proteomic Analysis of Exosomes and Exosome-Free Conditioned Media From Human Osteosarcoma Cell Lines Reveals Secretion of Proteins Related to Tumor Progression

Sofía Jerez, Héctor Araya, Roman Thaler, M. Cristine Charlesworth, Remigio López-Solís, Alexis M. Kalergis, Pablo F. Céspedes, Amel Dudakovic, Gary S. Stein, Andre J van Wijnen, Mario Galindo

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Osteosarcomas are the most prevalent bone tumors in pediatric patients, but can also occur later in life. Bone tumors have the potential to metastasize to lung and occasionally other vital organs. To understand how osteosarcoma cells interact with their micro-environment to support bone tumor progression and metastasis, we analyzed secreted proteins and exosomes from three human osteosarcoma cell lines. Exosome isolation was validated by transmission electron microscopy (TEM) and immuno-blotting for characteristic biomarkers (CD63, CD9, and CD81). Exosomal and soluble proteins (less than 100 kDa) were identified by mass spectrometry analysis using nanoLC-MS/MS and classified by functional gene ontology clustering. We identified a secretome set of >3,000 proteins for both fractions, and detected proteins that are either common or unique among the three osteosarcoma cell lines. Protein ontology comparison of proteomes from exosomes and exosome-free fractions revealed differences in the enrichment of functional categories associated with different biological processes, including those related to tumor progression (i.e., angiogenesis, cell adhesion, and cell migration). The secretome characteristics of osteosarcoma cells are consistent with the pathological properties of tumor cells with metastatic potential. J. Cell. Biochem. 118: 351–360, 2017.

Original languageEnglish (US)
Pages (from-to)351-360
Number of pages10
JournalJournal of Cellular Biochemistry
Volume118
Issue number2
DOIs
StatePublished - Feb 1 2017

Fingerprint

Exosomes
Osteosarcoma
Conditioned Culture Medium
Proteomics
Tumors
Cells
Cell Line
Bone
Neoplasms
Proteins
Bone and Bones
Ontology
Biological Phenomena
Gene Ontology
Pediatrics
Cell adhesion
Biomarkers
Proteome
Transmission Electron Microscopy
Cell Adhesion

Keywords

  • CANCER
  • EXOSOME
  • OSTEOSARCOMA
  • SECRETOME
  • TUMOR PROGRESSION

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Proteomic Analysis of Exosomes and Exosome-Free Conditioned Media From Human Osteosarcoma Cell Lines Reveals Secretion of Proteins Related to Tumor Progression. / Jerez, Sofía; Araya, Héctor; Thaler, Roman; Charlesworth, M. Cristine; López-Solís, Remigio; Kalergis, Alexis M.; Céspedes, Pablo F.; Dudakovic, Amel; Stein, Gary S.; van Wijnen, Andre J; Galindo, Mario.

In: Journal of Cellular Biochemistry, Vol. 118, No. 2, 01.02.2017, p. 351-360.

Research output: Contribution to journalArticle

Jerez, S, Araya, H, Thaler, R, Charlesworth, MC, López-Solís, R, Kalergis, AM, Céspedes, PF, Dudakovic, A, Stein, GS, van Wijnen, AJ & Galindo, M 2017, 'Proteomic Analysis of Exosomes and Exosome-Free Conditioned Media From Human Osteosarcoma Cell Lines Reveals Secretion of Proteins Related to Tumor Progression', Journal of Cellular Biochemistry, vol. 118, no. 2, pp. 351-360. https://doi.org/10.1002/jcb.25642
Jerez, Sofía ; Araya, Héctor ; Thaler, Roman ; Charlesworth, M. Cristine ; López-Solís, Remigio ; Kalergis, Alexis M. ; Céspedes, Pablo F. ; Dudakovic, Amel ; Stein, Gary S. ; van Wijnen, Andre J ; Galindo, Mario. / Proteomic Analysis of Exosomes and Exosome-Free Conditioned Media From Human Osteosarcoma Cell Lines Reveals Secretion of Proteins Related to Tumor Progression. In: Journal of Cellular Biochemistry. 2017 ; Vol. 118, No. 2. pp. 351-360.
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