TY - JOUR
T1 - Proteomic analysis of an unsequenced plant - Mangifera indica
AU - Renuse, Santosh
AU - Harsha, H. C.
AU - Kumar, Praveen
AU - Acharya, Pradip Kumar
AU - Sharma, Jyoti
AU - Goel, Renu
AU - Kumar, Ghantasala S.Sameer
AU - Raju, Rajesh
AU - Prasad, T. S.Keshava
AU - Slotta, Tracey
AU - Pandey, Akhilesh
N1 - Funding Information:
We thank the Department of Biotechnology, Government of India for research support to the Institute of Bioinformatics. Santosh Renuse is a recipient of a Senior Research fellowship from the University Grants Commission (UGC) of the Government of India . Harsha Gowda is a Wellcome Trust/DBT India Alliance Early Career Fellow. Jyoti Sharma and Rajesh Raju are recipients of Senior Research fellowship award from Council of Scientific and Industrial Research (CSIR), Government of India. Thottethodi Subrahmanya Keshava Prasad is a recipient of Young Investigator Award from Department of Biotechnology, Government of India. We acknowledge Roopashree Subbaiah and Harsh Pawar for their assistance in sample preparation. We thank Agilent Technologies for access to instrumentation.
PY - 2012/10/22
Y1 - 2012/10/22
N2 - Mangifera indica (Mango) is an important fruit crop in tropical countries with India being the leading producer in the world. Substantial research efforts are being devoted to produce fruit that have desirable characteristics including those that pertain to taste, hardiness and resistance to pests. Characterization of the genome and proteome of mango would help in the improvement of cultivars. As the mango genome has not yet been sequenced, we employed a mass spectrometry-based approach followed by database searches of mango-derived ESTs and proteins along with proteins from six other closely related plant species to characterize its proteome. In addition to this, de novo sequencing followed by homology-based protein identification was also carried out. The LC-MS/MS analysis of the mango leaf proteome was performed using an accurate mass quadrupole time-of-flight mass spectrometer. This integrative approach enabled the identification of 1001 peptides that matched to 538 proteins. To our knowledge, this study is the first high-throughput analysis of mango leaf proteome and could pave the way for further genomic, transcriptomic and proteomic studies.
AB - Mangifera indica (Mango) is an important fruit crop in tropical countries with India being the leading producer in the world. Substantial research efforts are being devoted to produce fruit that have desirable characteristics including those that pertain to taste, hardiness and resistance to pests. Characterization of the genome and proteome of mango would help in the improvement of cultivars. As the mango genome has not yet been sequenced, we employed a mass spectrometry-based approach followed by database searches of mango-derived ESTs and proteins along with proteins from six other closely related plant species to characterize its proteome. In addition to this, de novo sequencing followed by homology-based protein identification was also carried out. The LC-MS/MS analysis of the mango leaf proteome was performed using an accurate mass quadrupole time-of-flight mass spectrometer. This integrative approach enabled the identification of 1001 peptides that matched to 538 proteins. To our knowledge, this study is the first high-throughput analysis of mango leaf proteome and could pave the way for further genomic, transcriptomic and proteomic studies.
KW - Mango
KW - Mass spectrometry
KW - Plant proteomics
KW - QTOF
UR - http://www.scopus.com/inward/record.url?scp=84866172199&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84866172199&partnerID=8YFLogxK
U2 - 10.1016/j.jprot.2012.08.003
DO - 10.1016/j.jprot.2012.08.003
M3 - Article
C2 - 22906602
AN - SCOPUS:84866172199
SN - 1874-3919
VL - 75
SP - 5793
EP - 5796
JO - Journal of Proteomics
JF - Journal of Proteomics
IS - 18
ER -