Proteome profiling of wild type and lumican-deficient mouse corneas

Han Juan Shao, Raghothama Chaerkady, Shoujun Chen, Sneha M. Pinto, Rakesh Sharma, Bernard Delanghe, David E. Birk, Akhilesh Pandey, Shukti Chakravarti

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

To elucidate how the deficiency of a major corneal proteoglycan, lumican, affects corneal homeostasis, we used mass spectrometry to derive the proteome profile of the lumican-deficient and the heterozygous mouse corneas and compared these to the wild type corneal proteome. 2108 proteins were quantified in the mouse cornea. Selected proteins and transcripts were investigated by Western blot and quantitative RT-PCR, respectively. We observed major changes in the composition of the stromal extracellular matrix (ECM) proteins in the lumican-deficient mice. Lumican deficiency altered cellular proteins in the stroma and the corneal epithelium. The ECM changes included increases in fibril forming collagen type I, Collagen type VI, fibromodulin, perlecan, laminin β 2 , collagen type IV, nidogen/entactin and anchoring collagen type VII in the Lum +/- and the Lum -/- mouse corneas, while the stromal proteoglycans decorin, biglycan and keratocan were decreased in the Lum -/- corneas. Cellular protein changes included increases in alcohol dehydrogenase, superoxide dismutase and decreases in epithelial cytokeratins 8 and 14. We also detected proteins that are novel to the cornea. The proteomes will provide an insight into the lumican-deficient corneal phenotype of stromal thinning and loss of transparency and a better understanding of pathogenic changes in corneal and ocular dystrophies.

Original languageEnglish (US)
Pages (from-to)1895-1905
Number of pages11
JournalJournal of Proteomics
Volume74
Issue number10
DOIs
StatePublished - Sep 6 2011
Externally publishedYes

Fingerprint

Proteome
Cornea
Proteoglycans
Proteins
Collagen Type VII
Collagen Type VI
Biglycan
Keratin-14
Keratin-8
Decorin
Corneal Epithelium
Collagen Type IV
Alcohol Dehydrogenase
Extracellular Matrix Proteins
Laminin
Collagen Type I
Transparency
Superoxide Dismutase
Extracellular Matrix
Mass spectrometry

Keywords

  • Collagen
  • Cornea
  • ITRAQ
  • Lumican
  • Mass spectrometry
  • Proteomics

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry

Cite this

Shao, H. J., Chaerkady, R., Chen, S., Pinto, S. M., Sharma, R., Delanghe, B., ... Chakravarti, S. (2011). Proteome profiling of wild type and lumican-deficient mouse corneas. Journal of Proteomics, 74(10), 1895-1905. https://doi.org/10.1016/j.jprot.2011.04.032

Proteome profiling of wild type and lumican-deficient mouse corneas. / Shao, Han Juan; Chaerkady, Raghothama; Chen, Shoujun; Pinto, Sneha M.; Sharma, Rakesh; Delanghe, Bernard; Birk, David E.; Pandey, Akhilesh; Chakravarti, Shukti.

In: Journal of Proteomics, Vol. 74, No. 10, 06.09.2011, p. 1895-1905.

Research output: Contribution to journalArticle

Shao, HJ, Chaerkady, R, Chen, S, Pinto, SM, Sharma, R, Delanghe, B, Birk, DE, Pandey, A & Chakravarti, S 2011, 'Proteome profiling of wild type and lumican-deficient mouse corneas', Journal of Proteomics, vol. 74, no. 10, pp. 1895-1905. https://doi.org/10.1016/j.jprot.2011.04.032
Shao HJ, Chaerkady R, Chen S, Pinto SM, Sharma R, Delanghe B et al. Proteome profiling of wild type and lumican-deficient mouse corneas. Journal of Proteomics. 2011 Sep 6;74(10):1895-1905. https://doi.org/10.1016/j.jprot.2011.04.032
Shao, Han Juan ; Chaerkady, Raghothama ; Chen, Shoujun ; Pinto, Sneha M. ; Sharma, Rakesh ; Delanghe, Bernard ; Birk, David E. ; Pandey, Akhilesh ; Chakravarti, Shukti. / Proteome profiling of wild type and lumican-deficient mouse corneas. In: Journal of Proteomics. 2011 ; Vol. 74, No. 10. pp. 1895-1905.
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