Proteolytic processing of the Ebola virus glycoprotein is not critical for Ebola virus replication in nonhuman primates

Gabriele Neumann; Thomas W. Geisbert; Hideki Ebihara; Joan B. Geisbert; Kathleen M. Daddario-DiCaprio; Heinz Feldmann; Yoshihiro Kawaoka

doi:10.1128/JVI.02486-06

Proteolytic processing of the Ebola virus glycoprotein is not critical for Ebola virus replication in nonhuman primates

Gabriele Neumann, Thomas W. Geisbert, Hideki Ebihara, Joan B. Geisbert, Kathleen M. Daddario-DiCaprio, Heinz Feldmann, Yoshihiro Kawaoka

Molecular Medicine

Research output: Contribution to journal › Article › peer-review

42 Scopus citations

Abstract

Enveloped viruses often require cleavage of a surface glycoprotein by a cellular endoprotease such as furin for infectivity and virulence. Previously, we showed that Ebola virus glycoprotein does not require the furin cleavage motif for virus replication in cell culture. Here, we show that there are no appreciable differences in disease progression, hematology, serum biochemistry, virus titers, or lethality in nonhuman primates infected with an Ebola virus lacking the furin recognition sequence compared to those infected with wild-type virus. We conclude that glycoprotein cleavage by subtilisin-like endoproteases is not critical for Ebola virus infectivity and virulence in nonhuman primates.

Original language	English (US)
Pages (from-to)	2995-2998
Number of pages	4
Journal	Journal of virology
Volume	81
Issue number	6
DOIs	https://doi.org/10.1128/JVI.02486-06
State	Published - Mar 2007

ASJC Scopus subject areas

Microbiology
Immunology
Insect Science
Virology

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abstract = "Enveloped viruses often require cleavage of a surface glycoprotein by a cellular endoprotease such as furin for infectivity and virulence. Previously, we showed that Ebola virus glycoprotein does not require the furin cleavage motif for virus replication in cell culture. Here, we show that there are no appreciable differences in disease progression, hematology, serum biochemistry, virus titers, or lethality in nonhuman primates infected with an Ebola virus lacking the furin recognition sequence compared to those infected with wild-type virus. We conclude that glycoprotein cleavage by subtilisin-like endoproteases is not critical for Ebola virus infectivity and virulence in nonhuman primates.",

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AU - Neumann, Gabriele

AU - Geisbert, Thomas W.

AU - Ebihara, Hideki

AU - Geisbert, Joan B.

AU - Daddario-DiCaprio, Kathleen M.

AU - Feldmann, Heinz

AU - Kawaoka, Yoshihiro

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AB - Enveloped viruses often require cleavage of a surface glycoprotein by a cellular endoprotease such as furin for infectivity and virulence. Previously, we showed that Ebola virus glycoprotein does not require the furin cleavage motif for virus replication in cell culture. Here, we show that there are no appreciable differences in disease progression, hematology, serum biochemistry, virus titers, or lethality in nonhuman primates infected with an Ebola virus lacking the furin recognition sequence compared to those infected with wild-type virus. We conclude that glycoprotein cleavage by subtilisin-like endoproteases is not critical for Ebola virus infectivity and virulence in nonhuman primates.

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Proteolytic processing of the Ebola virus glycoprotein is not critical for Ebola virus replication in nonhuman primates

Abstract

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