Proteins of chromatin in template restriction. III. The macromolecules in specific restriction of the chromatin DNA

T. C. Spelsberg; L. S. Hnilica; A. T. Ansevin

doi:10.1016/0005-2787(71)90061-X

Proteins of chromatin in template restriction. III. The macromolecules in specific restriction of the chromatin DNA

T. C. Spelsberg, L. S. Hnilica, A. T. Ansevin

Biochemistry and Molecular Biology

Research output: Contribution to journal › Article › peer-review

159 Scopus citations

Abstract

Macromolecules influencing the tissue-specific restriction of DNA in chromatin from rat liver and thymus were studied. It was found that the specificity of RNA synthesis in vitro (as detected by DNA-RNA hybridization) can be changed from the pattern characteristic for rat liver to that of rat thymus and vice versa by exchanging the nonhistone proteins associated with the DNA in chromatin and reconstituting this fraction back to the DNA and histones by gradient dialysis. The origin of the histones did not contribute to the specificity of DNA restriction in isolated and reconstituted chromatin. Even the reconstitution of calf thymus histones to dehistonized rat liver chromatin did not alter the specificity of RNA normally synthesized by rat liver chromatin templates.

Original language	English (US)
Pages (from-to)	550-562
Number of pages	13
Journal	BBA Section Nucleic Acids And Protein Synthesis
Volume	228
Issue number	2
DOIs	https://doi.org/10.1016/0005-2787(71)90061-X
State	Published - Jan 28 1971

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General Medicine

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title = "Proteins of chromatin in template restriction. III. The macromolecules in specific restriction of the chromatin DNA",

abstract = "Macromolecules influencing the tissue-specific restriction of DNA in chromatin from rat liver and thymus were studied. It was found that the specificity of RNA synthesis in vitro (as detected by DNA-RNA hybridization) can be changed from the pattern characteristic for rat liver to that of rat thymus and vice versa by exchanging the nonhistone proteins associated with the DNA in chromatin and reconstituting this fraction back to the DNA and histones by gradient dialysis. The origin of the histones did not contribute to the specificity of DNA restriction in isolated and reconstituted chromatin. Even the reconstitution of calf thymus histones to dehistonized rat liver chromatin did not alter the specificity of RNA normally synthesized by rat liver chromatin templates.",

author = "Spelsberg, {T. C.} and Hnilica, {L. S.} and Ansevin, {A. T.}",

note = "Funding Information: The authors wish to acknowledge their appreciation of the excellent technical assistance of Miss Susan Getz and Miss Susan Kehm. This research was supported by the American Cancer Society Grant E-388, U.S. Public Health Service Grant CA-o7746 and the Robert A. Welch Foundation Grants G-I38 and 29 o. Computer processing of data was supported by the U.S. Public Health Service Grant CA 1143o to the Department of Biomathematics of The University of Texas M. D. Anderson Hospital and Tumor Institute at Houston.",

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doi = "10.1016/0005-2787(71)90061-X",

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T1 - Proteins of chromatin in template restriction. III. The macromolecules in specific restriction of the chromatin DNA

AU - Spelsberg, T. C.

AU - Hnilica, L. S.

AU - Ansevin, A. T.

N1 - Funding Information: The authors wish to acknowledge their appreciation of the excellent technical assistance of Miss Susan Getz and Miss Susan Kehm. This research was supported by the American Cancer Society Grant E-388, U.S. Public Health Service Grant CA-o7746 and the Robert A. Welch Foundation Grants G-I38 and 29 o. Computer processing of data was supported by the U.S. Public Health Service Grant CA 1143o to the Department of Biomathematics of The University of Texas M. D. Anderson Hospital and Tumor Institute at Houston.

PY - 1971/1/28

Y1 - 1971/1/28

N2 - Macromolecules influencing the tissue-specific restriction of DNA in chromatin from rat liver and thymus were studied. It was found that the specificity of RNA synthesis in vitro (as detected by DNA-RNA hybridization) can be changed from the pattern characteristic for rat liver to that of rat thymus and vice versa by exchanging the nonhistone proteins associated with the DNA in chromatin and reconstituting this fraction back to the DNA and histones by gradient dialysis. The origin of the histones did not contribute to the specificity of DNA restriction in isolated and reconstituted chromatin. Even the reconstitution of calf thymus histones to dehistonized rat liver chromatin did not alter the specificity of RNA normally synthesized by rat liver chromatin templates.

AB - Macromolecules influencing the tissue-specific restriction of DNA in chromatin from rat liver and thymus were studied. It was found that the specificity of RNA synthesis in vitro (as detected by DNA-RNA hybridization) can be changed from the pattern characteristic for rat liver to that of rat thymus and vice versa by exchanging the nonhistone proteins associated with the DNA in chromatin and reconstituting this fraction back to the DNA and histones by gradient dialysis. The origin of the histones did not contribute to the specificity of DNA restriction in isolated and reconstituted chromatin. Even the reconstitution of calf thymus histones to dehistonized rat liver chromatin did not alter the specificity of RNA normally synthesized by rat liver chromatin templates.

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Proteins of chromatin in template restriction. III. The macromolecules in specific restriction of the chromatin DNA

Abstract

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