Proteins of chromatin in template restriction. II. Specificity of RNA synthesis

Thomas C. Spelsberg; L. S. Hnilica

doi:10.1016/0005-2787(71)90561-2

Proteins of chromatin in template restriction. II. Specificity of RNA synthesis

Thomas C. Spelsberg, L. S. Hnilica

Biochemistry and Molecular Biology

Research output: Contribution to journal › Article › peer-review

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Abstract

Selective dissociation of histones from rat thymus chromatin resulted in a gradual loss of the specificity of DNA template restriction as shown by rate studies of the in vitro RNA synthesis and by DNA-RNA hybridization. Removal of the very lysine-rich histones F1 did not change the templating properties of chromatin. Dissociation of either the moderately lysine-rich histone F2b or the arginine-rich fractions F2a and F3 derepressed the DNA only partially. Even after the removal of practically all histones and of a part (approx. 20 %) of the nonhistone proteins, significant template restriction was retained in the treated chromatin.

Original language	English (US)
Pages (from-to)	212-222
Number of pages	11
Journal	BBA Section Nucleic Acids And Protein Synthesis
Volume	228
Issue number	1
DOIs	https://doi.org/10.1016/0005-2787(71)90561-2
State	Published - Jan 1 1971

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General Medicine

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title = "Proteins of chromatin in template restriction. II. Specificity of RNA synthesis",

abstract = "Selective dissociation of histones from rat thymus chromatin resulted in a gradual loss of the specificity of DNA template restriction as shown by rate studies of the in vitro RNA synthesis and by DNA-RNA hybridization. Removal of the very lysine-rich histones F1 did not change the templating properties of chromatin. Dissociation of either the moderately lysine-rich histone F2b or the arginine-rich fractions F2a and F3 derepressed the DNA only partially. Even after the removal of practically all histones and of a part (approx. 20 %) of the nonhistone proteins, significant template restriction was retained in the treated chromatin.",

author = "Spelsberg, {Thomas C.} and Hnilica, {L. S.}",

note = "Funding Information: Appreciationi s extendedt o Dr. John A. Wilhelm for his advice and criticism in the preparationo f this manuscript.T he experimentawl ork was supportedb y an American Cancer SocietyG rant No. E388, U.S. Public Health ServiceG rant No. CA-o7746, The Robert A. Welch Foundation Grant No. G-I38, and an Institutional Grant No. FR 55II-IN-87 . Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",

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AU - Hnilica, L. S.

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N2 - Selective dissociation of histones from rat thymus chromatin resulted in a gradual loss of the specificity of DNA template restriction as shown by rate studies of the in vitro RNA synthesis and by DNA-RNA hybridization. Removal of the very lysine-rich histones F1 did not change the templating properties of chromatin. Dissociation of either the moderately lysine-rich histone F2b or the arginine-rich fractions F2a and F3 derepressed the DNA only partially. Even after the removal of practically all histones and of a part (approx. 20 %) of the nonhistone proteins, significant template restriction was retained in the treated chromatin.

AB - Selective dissociation of histones from rat thymus chromatin resulted in a gradual loss of the specificity of DNA template restriction as shown by rate studies of the in vitro RNA synthesis and by DNA-RNA hybridization. Removal of the very lysine-rich histones F1 did not change the templating properties of chromatin. Dissociation of either the moderately lysine-rich histone F2b or the arginine-rich fractions F2a and F3 derepressed the DNA only partially. Even after the removal of practically all histones and of a part (approx. 20 %) of the nonhistone proteins, significant template restriction was retained in the treated chromatin.

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Proteins of chromatin in template restriction. II. Specificity of RNA synthesis

Abstract

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