Proteins of chromatin in template restriction. II. Specificity of RNA synthesis

Thomas C. Spelsberg, L. S. Hnilica

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Selective dissociation of histones from rat thymus chromatin resulted in a gradual loss of the specificity of DNA template restriction as shown by rate studies of the in vitro RNA synthesis and by DNA-RNA hybridization. Removal of the very lysine-rich histones F1 did not change the templating properties of chromatin. Dissociation of either the moderately lysine-rich histone F2b or the arginine-rich fractions F2a and F3 derepressed the DNA only partially. Even after the removal of practically all histones and of a part (approx. 20 %) of the nonhistone proteins, significant template restriction was retained in the treated chromatin.

Original languageEnglish (US)
Pages (from-to)212-222
Number of pages11
JournalBBA Section Nucleic Acids And Protein Synthesis
Volume228
Issue number1
DOIs
StatePublished - Jan 1 1971
Externally publishedYes

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Histones
Chromatin
RNA
Lysine
DNA
Proteins
Thymus Gland
Arginine

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Proteins of chromatin in template restriction. II. Specificity of RNA synthesis. / Spelsberg, Thomas C.; Hnilica, L. S.

In: BBA Section Nucleic Acids And Protein Synthesis, Vol. 228, No. 1, 01.01.1971, p. 212-222.

Research output: Contribution to journalArticle

Spelsberg, Thomas C. ; Hnilica, L. S. / Proteins of chromatin in template restriction. II. Specificity of RNA synthesis. In: BBA Section Nucleic Acids And Protein Synthesis. 1971 ; Vol. 228, No. 1. pp. 212-222.
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