Proteinase-activated receptors, targets for kallikrein signaling

Katerina Oikonomopoulou, Kristina K. Hansen, Mahmoud Saifeddine, Illa Tea, Michael Blaber, Sachiko I. Blaber, Isobel A Scarisbrick, Patricia Andrade-Gordon, Graeme S. Cottrell, Nigel W. Bunnett, Eleftherios P. Diamandis, Morley D. Hollenberg

Research output: Contribution to journalArticle

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Abstract

Serine proteinases like thrombin can signal to cells by the cleavage/activation of proteinase-activated receptors (PARs). Although thrombin is a recognized physiological activator of PAR1 and PAR4, the endogenous enzymes responsible for activating PAR2 in settings other than the gastrointestinal system, where trypsin can activate PAR 2, are unknown. We tested the hypothesis that the human tissue kallikrein (hK) family of proteinases regulates PAR signaling by using the following: 1) a high pressure liquid chromatography (HPLC)-mass spectral analysis of the cleavage products yielded upon incubation of hK5, -6, and -14 with synthetic PAR N-terminal peptide sequences representing the cleavage/activation motifs of PAR1, PAR2, and PAR 4; 2) PAR-dependent calcium signaling responses in cells expressing PAR1, PAR2, and PAR4 and in human platelets; 3) a vascular ring vasorelaxation assay; and 4) a PAR4-dependent rat and human platelet aggregation assay. We found that hK5, -6, and -14 all yielded PAR peptide cleavage sequences consistent with either receptor activation or inactivation/disarming. Furthermore, hK14 was able to activate PAR1, PAR2, and PAR4 and to disarm/inhibit PAR1. Although hK5 and -6 were also able to activate PAR2, they failed to cause PAR4-dependent aggregation of rat and human platelets, although hK14 did. Furthermore, the relative potencies and maximum effects of hK14 and -6 to activate PAR2-mediated calcium signaling differed. Our data indicate that in physiological settings, hKs may represent important endogenous regulators of the PARs and that different hKs can have differential actions on PAR1, PAR2, and PAR4.

Original languageEnglish (US)
Pages (from-to)32095-32112
Number of pages18
JournalJournal of Biological Chemistry
Volume281
Issue number43
DOIs
StatePublished - Oct 27 2006

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Proteinase-Activated Receptors
Kallikreins
Platelets
PAR-2 Receptor
Calcium Signaling
Chemical activation
Thrombin
Rats
Assays
Blood Platelets
Agglomeration
High pressure liquid chromatography
Calcium
Tissue Kallikreins
Peptides
Serine Proteases
Platelet Aggregation
Vasodilation
Spectrum analysis
Trypsin

ASJC Scopus subject areas

  • Biochemistry

Cite this

Oikonomopoulou, K., Hansen, K. K., Saifeddine, M., Tea, I., Blaber, M., Blaber, S. I., ... Hollenberg, M. D. (2006). Proteinase-activated receptors, targets for kallikrein signaling. Journal of Biological Chemistry, 281(43), 32095-32112. https://doi.org/10.1074/jbc.M513138200

Proteinase-activated receptors, targets for kallikrein signaling. / Oikonomopoulou, Katerina; Hansen, Kristina K.; Saifeddine, Mahmoud; Tea, Illa; Blaber, Michael; Blaber, Sachiko I.; Scarisbrick, Isobel A; Andrade-Gordon, Patricia; Cottrell, Graeme S.; Bunnett, Nigel W.; Diamandis, Eleftherios P.; Hollenberg, Morley D.

In: Journal of Biological Chemistry, Vol. 281, No. 43, 27.10.2006, p. 32095-32112.

Research output: Contribution to journalArticle

Oikonomopoulou, K, Hansen, KK, Saifeddine, M, Tea, I, Blaber, M, Blaber, SI, Scarisbrick, IA, Andrade-Gordon, P, Cottrell, GS, Bunnett, NW, Diamandis, EP & Hollenberg, MD 2006, 'Proteinase-activated receptors, targets for kallikrein signaling', Journal of Biological Chemistry, vol. 281, no. 43, pp. 32095-32112. https://doi.org/10.1074/jbc.M513138200
Oikonomopoulou K, Hansen KK, Saifeddine M, Tea I, Blaber M, Blaber SI et al. Proteinase-activated receptors, targets for kallikrein signaling. Journal of Biological Chemistry. 2006 Oct 27;281(43):32095-32112. https://doi.org/10.1074/jbc.M513138200
Oikonomopoulou, Katerina ; Hansen, Kristina K. ; Saifeddine, Mahmoud ; Tea, Illa ; Blaber, Michael ; Blaber, Sachiko I. ; Scarisbrick, Isobel A ; Andrade-Gordon, Patricia ; Cottrell, Graeme S. ; Bunnett, Nigel W. ; Diamandis, Eleftherios P. ; Hollenberg, Morley D. / Proteinase-activated receptors, targets for kallikrein signaling. In: Journal of Biological Chemistry. 2006 ; Vol. 281, No. 43. pp. 32095-32112.
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AU - Blaber, Sachiko I.

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AU - Diamandis, Eleftherios P.

AU - Hollenberg, Morley D.

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