@inbook{98cf33e526584d45a60b67b83dc397ff,
title = "Protein stability in the presence of cosolutes",
abstract = "Protein scientists have long used cosolutes to study protein stability. While denaturants, such as urea, have been employed for a long time, the attention became focused more recently on protein stabilizers, including osmolytes. Here, we provide practical experimental instructions for the use of both stabilizing and denaturing osmolytes with proteins, as well as data evaluation strategies. We focus on protein stability in the presence of cosolutes and their mixtures at constant and variable temperature.",
keywords = "Folding, Osmolyte, Protein phase diagrams, Protein stability, Urea, m-values",
author = "Holthauzen, {Luis Marcelo F.} and Matthew Auton and Mikhail Sinev and J{\"o}rg R{\"o}sgen",
note = "Funding Information: The authors gratefully acknowledge support by NIH through grant GM049760 to J. R.",
year = "2011",
doi = "10.1016/B978-0-12-381268-1.00015-X",
language = "English (US)",
series = "Methods in Enzymology",
publisher = "Academic Press Inc",
pages = "61--125",
booktitle = "Methods in Enzymology",
}