Protein-mediated autoreduction of gold salts to gold nanoparticles

Nivedita Basu, Resham Bhattacharya, Priyabrata Mukherjee

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Here we report for the first time that proteins can function as unique reducing agents to produce gold nanoparticles from gold salts. We demonstrate that three different proteins, namely, bovine serum albumin (BSA), Rituximab (RIT - an anti-CD20 antibody) and Cetuximab (C225 - anti-EGFR antibody), reduce gold salts to gold nanoparticles (GNP). Interestingly, among all the three proteins tested, only BSA can reduce gold salts to gold nanotriangles (GNT). BSA-induced formation of GNT can be controlled by carefully selecting the reaction condition. Heating or using excess of ascorbic acid (AA) as additional reducing agent shifts the reaction towards the formation of GNP with flower-like morphology, whereas slowing down the reaction either by cooling or by adding small amount of AA directs the synthesis towards GNT formation. GNT is formed only at pH 3; higher pHs (pH 7 and pH 10) did not produce any nanoparticles, suggesting the involvement of specific protein conformation in GNT formation. The nanomaterials formed by this method were characterized using UV-visible (UV-vis) spectroscopy and transmission electron microscopy (TEM). This is an important finding that will have uses in various nanotechnological applications, particularly in the green synthesis of novel nanomaterials based on protein structure.

Original languageEnglish (US)
Article number034105
JournalBiomedical Materials
Volume3
Issue number3
DOIs
StatePublished - Sep 1 2008

Fingerprint

Gold
Nanoparticles
Salts
Proteins
Bovine Serum Albumin
Nanostructures
Ascorbic acid
Reducing Agents
Reducing agents
Nanostructured materials
Antibodies
Ascorbic Acid
Anti-Idiotypic Antibodies
Protein Conformation
Ultraviolet spectroscopy
Transmission Electron Microscopy
Heating
Conformations
Spectrum Analysis
Transmission electron microscopy

ASJC Scopus subject areas

  • Chemistry (miscellaneous)
  • Biochemistry
  • Mechanics of Materials
  • Medicine(all)

Cite this

Protein-mediated autoreduction of gold salts to gold nanoparticles. / Basu, Nivedita; Bhattacharya, Resham; Mukherjee, Priyabrata.

In: Biomedical Materials, Vol. 3, No. 3, 034105, 01.09.2008.

Research output: Contribution to journalArticle

Basu, Nivedita ; Bhattacharya, Resham ; Mukherjee, Priyabrata. / Protein-mediated autoreduction of gold salts to gold nanoparticles. In: Biomedical Materials. 2008 ; Vol. 3, No. 3.
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