Protein kinase D1-mediated phosphorylations regulate Vasodilator-stimulated phosphoprotein (VASP) localization and cell migration

Heike R. Döppler, Ligia I. Bastea, Laura J. Lewis-Tuffin, Panagiotis Z Anastasiadis, Peter Storz

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Background: Protein kinase D1 (PKD1) regulates actin reorganization processes at the leading edge. Results: PKD1 phosphorylates VASP at two serine residues, Ser-157 and Ser-322. Conclusion: VASP is a substrate for PKD1. Significance: We provide an additional mechanism of how VASP functions can be regulated. Enabled/Vasodilator-stimulated phosphoprotein (Ena/VASP) protein family members link actin dynamics and cellular signaling pathways. VASP localizes to regions of dynamic actin reorganization such as the focal adhesion contacts, the leading edge or filopodia, where it contributes to F-actin filament elongation. Here we identify VASP as a novel substrate for protein kinase D1 (PKD1). We show that PKD1 directly phosphorylates VASP at two serine residues, Ser-157 and Ser-322. These phosphorylations occur in response to RhoA activation and mediate VASP re-localization from focal contacts to the leading edge region. The net result of this PKD1-mediated phosphorylation switch in VASP is increased filopodia formation and length at the leading edge. However, such signaling when persistent induced membrane ruffling and decreased cell motility.

Original languageEnglish (US)
Pages (from-to)24382-24393
Number of pages12
JournalJournal of Biological Chemistry
Volume288
Issue number34
DOIs
StatePublished - Aug 23 2013

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Phosphorylation
Protein Kinases
Cell Movement
Focal Adhesions
Actins
Pseudopodia
Serine
Cell signaling
vasodilator-stimulated phosphoprotein
Substrates
Actin Cytoskeleton
Elongation
Adhesion
Chemical activation
Switches
Membranes

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Protein kinase D1-mediated phosphorylations regulate Vasodilator-stimulated phosphoprotein (VASP) localization and cell migration. / Döppler, Heike R.; Bastea, Ligia I.; Lewis-Tuffin, Laura J.; Anastasiadis, Panagiotis Z; Storz, Peter.

In: Journal of Biological Chemistry, Vol. 288, No. 34, 23.08.2013, p. 24382-24393.

Research output: Contribution to journalArticle

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