Protein kinase C inhibition and activation phase advances the hamster circadian clock

Kathryn M. Schak; Mary E. Harrington

doi:10.1016/S0006-8993(99)01787-4

Protein kinase C inhibition and activation phase advances the hamster circadian clock

Kathryn M. Schak, Mary E. Harrington

Psychiatry and Psychology

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

The mammalian circadian clock is located in the suprachiasmatic nuclei (SCN). Clock function can be detected by the measurement of the circadian change in cellular firing rate of SCN cells in vitro. We investigated the effects of protein kinase C (PKC) inhibition and activation on this rhythm of firing rate in hamster SCN neurons. PKC inhibition by chelerythrine chloride application phase advances the in vitro circadian rhythm during the late subjective night and early subjective morning, Zeitgeber time (ZT) 20-24 and ZT 0-4. No effect of PKC inhibition on clock phase was seen during ZT 6-18. Activation of PKC via phorbol 12-myristate 13-acetate (PMA) phase advanced the clock at all phases tested. Thus, at some circadian phases both inhibition and activation of PKC can advance circadian rhythms.

Original language	English (US)
Pages (from-to)	158-161
Number of pages	4
Journal	Brain Research
Volume	840
Issue number	1-2
DOIs	https://doi.org/10.1016/S0006-8993(99)01787-4
State	Published - Sep 4 1999

Keywords

Chelerthyrine chloride
Circadian
Protein kinase C
Rhythm
Suprachiasmatic

ASJC Scopus subject areas

General Neuroscience
Molecular Biology
Clinical Neurology
Developmental Biology

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10.1016/S0006-8993(99)01787-4

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title = "Protein kinase C inhibition and activation phase advances the hamster circadian clock",

abstract = "The mammalian circadian clock is located in the suprachiasmatic nuclei (SCN). Clock function can be detected by the measurement of the circadian change in cellular firing rate of SCN cells in vitro. We investigated the effects of protein kinase C (PKC) inhibition and activation on this rhythm of firing rate in hamster SCN neurons. PKC inhibition by chelerythrine chloride application phase advances the in vitro circadian rhythm during the late subjective night and early subjective morning, Zeitgeber time (ZT) 20-24 and ZT 0-4. No effect of PKC inhibition on clock phase was seen during ZT 6-18. Activation of PKC via phorbol 12-myristate 13-acetate (PMA) phase advanced the clock at all phases tested. Thus, at some circadian phases both inhibition and activation of PKC can advance circadian rhythms.",

keywords = "Chelerthyrine chloride, Circadian, Protein kinase C, Rhythm, Suprachiasmatic",

author = "Schak, {Kathryn M.} and Harrington, {Mary E.}",

note = "Funding Information: This study was supported by NIH grant NS26496 (M.E.H.). The technical assistance of Jennifer L. Meyer is gratefully acknowledged. We would like to thank Chiaki Fukuhara for helpful discussions.",

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T1 - Protein kinase C inhibition and activation phase advances the hamster circadian clock

AU - Schak, Kathryn M.

AU - Harrington, Mary E.

N1 - Funding Information: This study was supported by NIH grant NS26496 (M.E.H.). The technical assistance of Jennifer L. Meyer is gratefully acknowledged. We would like to thank Chiaki Fukuhara for helpful discussions.

PY - 1999/9/4

Y1 - 1999/9/4

N2 - The mammalian circadian clock is located in the suprachiasmatic nuclei (SCN). Clock function can be detected by the measurement of the circadian change in cellular firing rate of SCN cells in vitro. We investigated the effects of protein kinase C (PKC) inhibition and activation on this rhythm of firing rate in hamster SCN neurons. PKC inhibition by chelerythrine chloride application phase advances the in vitro circadian rhythm during the late subjective night and early subjective morning, Zeitgeber time (ZT) 20-24 and ZT 0-4. No effect of PKC inhibition on clock phase was seen during ZT 6-18. Activation of PKC via phorbol 12-myristate 13-acetate (PMA) phase advanced the clock at all phases tested. Thus, at some circadian phases both inhibition and activation of PKC can advance circadian rhythms.

AB - The mammalian circadian clock is located in the suprachiasmatic nuclei (SCN). Clock function can be detected by the measurement of the circadian change in cellular firing rate of SCN cells in vitro. We investigated the effects of protein kinase C (PKC) inhibition and activation on this rhythm of firing rate in hamster SCN neurons. PKC inhibition by chelerythrine chloride application phase advances the in vitro circadian rhythm during the late subjective night and early subjective morning, Zeitgeber time (ZT) 20-24 and ZT 0-4. No effect of PKC inhibition on clock phase was seen during ZT 6-18. Activation of PKC via phorbol 12-myristate 13-acetate (PMA) phase advanced the clock at all phases tested. Thus, at some circadian phases both inhibition and activation of PKC can advance circadian rhythms.

KW - Chelerthyrine chloride

KW - Circadian

KW - Protein kinase C

KW - Rhythm

KW - Suprachiasmatic

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JO - Brain Research

JF - Brain Research

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ER -

Protein kinase C inhibition and activation phase advances the hamster circadian clock

Abstract

Keywords

ASJC Scopus subject areas

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