Protein kinase C inhibition and activation phase advances the hamster circadian clock

Kathryn M. Schak, Mary E. Harrington

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The mammalian circadian clock is located in the suprachiasmatic nuclei (SCN). Clock function can be detected by the measurement of the circadian change in cellular firing rate of SCN cells in vitro. We investigated the effects of protein kinase C (PKC) inhibition and activation on this rhythm of firing rate in hamster SCN neurons. PKC inhibition by chelerythrine chloride application phase advances the in vitro circadian rhythm during the late subjective night and early subjective morning, Zeitgeber time (ZT) 20-24 and ZT 0-4. No effect of PKC inhibition on clock phase was seen during ZT 6-18. Activation of PKC via phorbol 12-myristate 13-acetate (PMA) phase advanced the clock at all phases tested. Thus, at some circadian phases both inhibition and activation of PKC can advance circadian rhythms.

Original languageEnglish (US)
Pages (from-to)158-161
Number of pages4
JournalBrain Research
Volume840
Issue number1-2
DOIs
StatePublished - Sep 4 1999
Externally publishedYes

Fingerprint

Circadian Clocks
Cricetinae
Protein Kinase C
Suprachiasmatic Nucleus
Circadian Rhythm
Acetates
Neurons

Keywords

  • Chelerthyrine chloride
  • Circadian
  • Protein kinase C
  • Rhythm
  • Suprachiasmatic

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Protein kinase C inhibition and activation phase advances the hamster circadian clock. / Schak, Kathryn M.; Harrington, Mary E.

In: Brain Research, Vol. 840, No. 1-2, 04.09.1999, p. 158-161.

Research output: Contribution to journalArticle

Schak, Kathryn M. ; Harrington, Mary E. / Protein kinase C inhibition and activation phase advances the hamster circadian clock. In: Brain Research. 1999 ; Vol. 840, No. 1-2. pp. 158-161.
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