Protein kinase C μ selectively activates the mitogen-activated protein kinase (MAPK) p42 pathway

Angelika Hausser, Peter Storz, Susanne Hübner, Ilona Braendlin, Marina Martinez-Moya, Gisela Link, Franz Josef Johannes

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Here we show that human protein kinase C μ (PKCμ) activates the mitogen-activated protein kinase (MAPK). Transient expression of constitutive active PKCμ leads to an activation of Raf-1 kinase as demonstrated by in vitro phosphorylation of MAPK. PKCμ enhances transcriptional activity of a basal thymidine kinase promotor containing serum response elements (SREs) as shown by luciferase reporter gene assays. SRE driven gene activation by PKCμ is triggered by the Elk-1 ternary complex factor. PKCμ-mediated activation of SRE driven transcription can be inhibited by the MEK1 inhibitor PD98059. In contrast to the activation of the p42/ERK1 MAPK cascade, transient expression of constitutive active PKCμ does neither affect c-jun N-terminal kinase nor p38 MAPK.

Original languageEnglish (US)
Pages (from-to)39-44
Number of pages6
JournalFEBS Letters
Volume492
Issue number1-2
DOIs
StatePublished - Mar 9 2001
Externally publishedYes

Fingerprint

Mitogen-Activated Protein Kinase 1
Protein Kinase C
Serum Response Element
Mitogen-Activated Protein Kinases
Chemical activation
Ternary Complex Factors
Genes
Proto-Oncogene Proteins c-raf
Phosphorylation
Thymidine Kinase
JNK Mitogen-Activated Protein Kinases
p38 Mitogen-Activated Protein Kinases
Transcription
Luciferases
Reporter Genes
Transcriptional Activation
Assays
Proteins

Keywords

  • Mitogen-activated protein kinase
  • p42
  • Protein kinase C μ
  • Serum response element

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Hausser, A., Storz, P., Hübner, S., Braendlin, I., Martinez-Moya, M., Link, G., & Johannes, F. J. (2001). Protein kinase C μ selectively activates the mitogen-activated protein kinase (MAPK) p42 pathway. FEBS Letters, 492(1-2), 39-44. https://doi.org/10.1016/S0014-5793(01)02219-0

Protein kinase C μ selectively activates the mitogen-activated protein kinase (MAPK) p42 pathway. / Hausser, Angelika; Storz, Peter; Hübner, Susanne; Braendlin, Ilona; Martinez-Moya, Marina; Link, Gisela; Johannes, Franz Josef.

In: FEBS Letters, Vol. 492, No. 1-2, 09.03.2001, p. 39-44.

Research output: Contribution to journalArticle

Hausser, A, Storz, P, Hübner, S, Braendlin, I, Martinez-Moya, M, Link, G & Johannes, FJ 2001, 'Protein kinase C μ selectively activates the mitogen-activated protein kinase (MAPK) p42 pathway', FEBS Letters, vol. 492, no. 1-2, pp. 39-44. https://doi.org/10.1016/S0014-5793(01)02219-0
Hausser, Angelika ; Storz, Peter ; Hübner, Susanne ; Braendlin, Ilona ; Martinez-Moya, Marina ; Link, Gisela ; Johannes, Franz Josef. / Protein kinase C μ selectively activates the mitogen-activated protein kinase (MAPK) p42 pathway. In: FEBS Letters. 2001 ; Vol. 492, No. 1-2. pp. 39-44.
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