Protein database, human retinal pigment epithelium.

Karen A. West, Lin Yan, Karen Shadrach, Jian Sun, Azeem Hasan, Masaru Miyagi, John S. Crabb, Joe G. Hollyfield, Alan D Marmorstein, John W. Crabb

Research output: Contribution to journalArticle

50 Scopus citations

Abstract

The retinal pigment epithelium (RPE) is a single cell layer adjacent to the rod and cone photoreceptors that plays key roles in retinal physiology and the biochemistry of vision. RPE cells were isolated from normal adult human donor eyes, subcellular fractions were prepared, and proteins were fractionated by electrophoresis. Following in-gel proteolysis, proteins were identified by peptide sequencing using liquid chromatography tandem electrospray mass spectrometry and/or by peptide mass mapping using matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Preliminary analyses have identified 278 proteins and provide a starting point for building a database of the human RPE proteome.

Original languageEnglish (US)
Pages (from-to)37-49
Number of pages13
JournalMolecular & cellular proteomics : MCP
Volume2
Issue number1
StatePublished - Jan 2003
Externally publishedYes

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ASJC Scopus subject areas

  • Biochemistry

Cite this

West, K. A., Yan, L., Shadrach, K., Sun, J., Hasan, A., Miyagi, M., Crabb, J. S., Hollyfield, J. G., Marmorstein, A. D., & Crabb, J. W. (2003). Protein database, human retinal pigment epithelium. Molecular & cellular proteomics : MCP, 2(1), 37-49.