Protein database, human retinal pigment epithelium.

Karen A. West, Lin Yan, Karen Shadrach, Jian Sun, Azeem Hasan, Masaru Miyagi, John S. Crabb, Joe G. Hollyfield, Alan D Marmorstein, John W. Crabb

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

The retinal pigment epithelium (RPE) is a single cell layer adjacent to the rod and cone photoreceptors that plays key roles in retinal physiology and the biochemistry of vision. RPE cells were isolated from normal adult human donor eyes, subcellular fractions were prepared, and proteins were fractionated by electrophoresis. Following in-gel proteolysis, proteins were identified by peptide sequencing using liquid chromatography tandem electrospray mass spectrometry and/or by peptide mass mapping using matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Preliminary analyses have identified 278 proteins and provide a starting point for building a database of the human RPE proteome.

Original languageEnglish (US)
Pages (from-to)37-49
Number of pages13
JournalMolecular & cellular proteomics : MCP
Volume2
Issue number1
StatePublished - Jan 2003
Externally publishedYes

Fingerprint

Protein Databases
Retinal Pigments
Retinal Pigment Epithelium
Mass spectrometry
Proteolysis
Retinal Cone Photoreceptor Cells
Retinal Rod Photoreceptor Cells
Peptides
Vertebrate Photoreceptor Cells
Biochemistry
Proteins
Peptide Mapping
Subcellular Fractions
Liquid chromatography
Physiology
Proteome
Tandem Mass Spectrometry
Electrophoresis
Liquid Chromatography
Ionization

ASJC Scopus subject areas

  • Biochemistry

Cite this

West, K. A., Yan, L., Shadrach, K., Sun, J., Hasan, A., Miyagi, M., ... Crabb, J. W. (2003). Protein database, human retinal pigment epithelium. Molecular & cellular proteomics : MCP, 2(1), 37-49.

Protein database, human retinal pigment epithelium. / West, Karen A.; Yan, Lin; Shadrach, Karen; Sun, Jian; Hasan, Azeem; Miyagi, Masaru; Crabb, John S.; Hollyfield, Joe G.; Marmorstein, Alan D; Crabb, John W.

In: Molecular & cellular proteomics : MCP, Vol. 2, No. 1, 01.2003, p. 37-49.

Research output: Contribution to journalArticle

West, KA, Yan, L, Shadrach, K, Sun, J, Hasan, A, Miyagi, M, Crabb, JS, Hollyfield, JG, Marmorstein, AD & Crabb, JW 2003, 'Protein database, human retinal pigment epithelium.', Molecular & cellular proteomics : MCP, vol. 2, no. 1, pp. 37-49.
West KA, Yan L, Shadrach K, Sun J, Hasan A, Miyagi M et al. Protein database, human retinal pigment epithelium. Molecular & cellular proteomics : MCP. 2003 Jan;2(1):37-49.
West, Karen A. ; Yan, Lin ; Shadrach, Karen ; Sun, Jian ; Hasan, Azeem ; Miyagi, Masaru ; Crabb, John S. ; Hollyfield, Joe G. ; Marmorstein, Alan D ; Crabb, John W. / Protein database, human retinal pigment epithelium. In: Molecular & cellular proteomics : MCP. 2003 ; Vol. 2, No. 1. pp. 37-49.
@article{c01099c886f6405fbce0818c63caad57,
title = "Protein database, human retinal pigment epithelium.",
abstract = "The retinal pigment epithelium (RPE) is a single cell layer adjacent to the rod and cone photoreceptors that plays key roles in retinal physiology and the biochemistry of vision. RPE cells were isolated from normal adult human donor eyes, subcellular fractions were prepared, and proteins were fractionated by electrophoresis. Following in-gel proteolysis, proteins were identified by peptide sequencing using liquid chromatography tandem electrospray mass spectrometry and/or by peptide mass mapping using matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Preliminary analyses have identified 278 proteins and provide a starting point for building a database of the human RPE proteome.",
author = "West, {Karen A.} and Lin Yan and Karen Shadrach and Jian Sun and Azeem Hasan and Masaru Miyagi and Crabb, {John S.} and Hollyfield, {Joe G.} and Marmorstein, {Alan D} and Crabb, {John W.}",
year = "2003",
month = "1",
language = "English (US)",
volume = "2",
pages = "37--49",
journal = "Molecular and Cellular Proteomics",
issn = "1535-9476",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "1",

}

TY - JOUR

T1 - Protein database, human retinal pigment epithelium.

AU - West, Karen A.

AU - Yan, Lin

AU - Shadrach, Karen

AU - Sun, Jian

AU - Hasan, Azeem

AU - Miyagi, Masaru

AU - Crabb, John S.

AU - Hollyfield, Joe G.

AU - Marmorstein, Alan D

AU - Crabb, John W.

PY - 2003/1

Y1 - 2003/1

N2 - The retinal pigment epithelium (RPE) is a single cell layer adjacent to the rod and cone photoreceptors that plays key roles in retinal physiology and the biochemistry of vision. RPE cells were isolated from normal adult human donor eyes, subcellular fractions were prepared, and proteins were fractionated by electrophoresis. Following in-gel proteolysis, proteins were identified by peptide sequencing using liquid chromatography tandem electrospray mass spectrometry and/or by peptide mass mapping using matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Preliminary analyses have identified 278 proteins and provide a starting point for building a database of the human RPE proteome.

AB - The retinal pigment epithelium (RPE) is a single cell layer adjacent to the rod and cone photoreceptors that plays key roles in retinal physiology and the biochemistry of vision. RPE cells were isolated from normal adult human donor eyes, subcellular fractions were prepared, and proteins were fractionated by electrophoresis. Following in-gel proteolysis, proteins were identified by peptide sequencing using liquid chromatography tandem electrospray mass spectrometry and/or by peptide mass mapping using matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Preliminary analyses have identified 278 proteins and provide a starting point for building a database of the human RPE proteome.

UR - http://www.scopus.com/inward/record.url?scp=0141560826&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0141560826&partnerID=8YFLogxK

M3 - Article

VL - 2

SP - 37

EP - 49

JO - Molecular and Cellular Proteomics

JF - Molecular and Cellular Proteomics

SN - 1535-9476

IS - 1

ER -