Protective role of major histocompatibility complex class II Ebd transgene on collagen-induced arthritis

Miguel A. Gonzalez-Gay, Gerald H. Nabozny, Michael J. Bull, Eric Zanelli, John Douhan, Marie M. Griffiths, Laurie H. Glimcher, Harvinder S. Luthra, Chella S. David

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Abstract

Collagen-induced arthritis (CIA) is an animal model of autoimmune inflammatory polyarthritis that has features similar to rheumatoid arthritis (RA). Much like RA, susceptibility to mouse CIA is influenced by the major histocompatibility complex (MHC), H-2, and restricted to the H-2q and H-2r haplotypes. Whereas the role of the H-2A molecule in susceptibility to CIA is well established, little is known about the role of H-2E molecule in the disease. In this study, we analyzed the effect of a transgenic Eβd molecule on CIA susceptibility in a recombinant mouse B10.RQB3, which expresses the CIA susceptible Aq genes and an Eak gene, but does not produce an E molecule since Ebq is nonfunctional. In the presence of an Ebd transgene, a viable E molecule is generated. Whereas B10.RQB3 were susceptible to CIA, B10.RQB3-Eβd+ showed a dramatic reduction in the incidence of arthritis as well as a decrease in the level of anti-mouse and anti-bovine CII antibodies in their serum. No clear cut differences in the expression of T cell receptor (TCR) Vβ was observed between Eβd+ and Eβd- transgenic mice. Mechanisms underlying the protective effect of Eβd transgenic molecule on CIA may shed light on how HLA-DR molecules influence human RA.

Original languageEnglish (US)
Pages (from-to)1559-1564
Number of pages6
JournalJournal of Experimental Medicine
Volume180
Issue number4
StatePublished - Oct 1 1994

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Experimental Arthritis
Major Histocompatibility Complex
Transgenes
Rheumatoid Arthritis
Arthritis
HLA-DR Antigens
T-Cell Antigen Receptor
Haplotypes
Transgenic Mice
Genes
Animal Models
Antibodies
Incidence
Serum

ASJC Scopus subject areas

  • Immunology

Cite this

Gonzalez-Gay, M. A., Nabozny, G. H., Bull, M. J., Zanelli, E., Douhan, J., Griffiths, M. M., ... David, C. S. (1994). Protective role of major histocompatibility complex class II Ebd transgene on collagen-induced arthritis. Journal of Experimental Medicine, 180(4), 1559-1564.

Protective role of major histocompatibility complex class II Ebd transgene on collagen-induced arthritis. / Gonzalez-Gay, Miguel A.; Nabozny, Gerald H.; Bull, Michael J.; Zanelli, Eric; Douhan, John; Griffiths, Marie M.; Glimcher, Laurie H.; Luthra, Harvinder S.; David, Chella S.

In: Journal of Experimental Medicine, Vol. 180, No. 4, 01.10.1994, p. 1559-1564.

Research output: Contribution to journalArticle

Gonzalez-Gay, MA, Nabozny, GH, Bull, MJ, Zanelli, E, Douhan, J, Griffiths, MM, Glimcher, LH, Luthra, HS & David, CS 1994, 'Protective role of major histocompatibility complex class II Ebd transgene on collagen-induced arthritis', Journal of Experimental Medicine, vol. 180, no. 4, pp. 1559-1564.
Gonzalez-Gay MA, Nabozny GH, Bull MJ, Zanelli E, Douhan J, Griffiths MM et al. Protective role of major histocompatibility complex class II Ebd transgene on collagen-induced arthritis. Journal of Experimental Medicine. 1994 Oct 1;180(4):1559-1564.
Gonzalez-Gay, Miguel A. ; Nabozny, Gerald H. ; Bull, Michael J. ; Zanelli, Eric ; Douhan, John ; Griffiths, Marie M. ; Glimcher, Laurie H. ; Luthra, Harvinder S. ; David, Chella S. / Protective role of major histocompatibility complex class II Ebd transgene on collagen-induced arthritis. In: Journal of Experimental Medicine. 1994 ; Vol. 180, No. 4. pp. 1559-1564.
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abstract = "Collagen-induced arthritis (CIA) is an animal model of autoimmune inflammatory polyarthritis that has features similar to rheumatoid arthritis (RA). Much like RA, susceptibility to mouse CIA is influenced by the major histocompatibility complex (MHC), H-2, and restricted to the H-2q and H-2r haplotypes. Whereas the role of the H-2A molecule in susceptibility to CIA is well established, little is known about the role of H-2E molecule in the disease. In this study, we analyzed the effect of a transgenic Eβd molecule on CIA susceptibility in a recombinant mouse B10.RQB3, which expresses the CIA susceptible Aq genes and an Eak gene, but does not produce an E molecule since Ebq is nonfunctional. In the presence of an Ebd transgene, a viable E molecule is generated. Whereas B10.RQB3 were susceptible to CIA, B10.RQB3-Eβd+ showed a dramatic reduction in the incidence of arthritis as well as a decrease in the level of anti-mouse and anti-bovine CII antibodies in their serum. No clear cut differences in the expression of T cell receptor (TCR) Vβ was observed between Eβd+ and Eβd- transgenic mice. Mechanisms underlying the protective effect of Eβd transgenic molecule on CIA may shed light on how HLA-DR molecules influence human RA.",
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AU - Griffiths, Marie M.

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