Protective effects of Puerariae flos against ethanol-induced apoptosis on human neuroblastoma cell line SK-N-MC

Mi-Hyeon D Jang, Min Chul Shin, Youn Jung Kim, Joo Ho Chung, Sung Vin Yim, Ee Hwa Kim, Yeunhee Kim, Chang Ju Kim

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Puerariae flos (PF) is an oriental medical herb for alcohol abuse. To investigate whether PF possesses protective effects against ethanol (EtOH)-induced cytotoxicity in the central nervous system, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, flow cytometric analysis, DNA fragmentation assay, and reverse transcription-polymerase chain reaction were performed on SK-N-MC human neuroblastoma cells. Cells treated with EtOH exhibited several apoptotic features, while those pre-treated with PF prior to EtOH exposure showed a decreased occurrence of apoptotic features. In addition, PF pre-treatment inhibited the EtOH-induced increase in caspase-3 mRNA expression. These results suggest that PF may exert protective effects against EtOH-induced apoptosis in human neuroblastoma cells.

Original languageEnglish (US)
Pages (from-to)338-342
Number of pages5
JournalJapanese Journal of Pharmacology
Volume87
Issue number4
DOIs
StatePublished - 2001
Externally publishedYes

Fingerprint

Pueraria
Neuroblastoma
Ethanol
Apoptosis
Cell Line
DNA Fragmentation
Caspase 3
Alcoholism
Reverse Transcription
Central Nervous System
Polymerase Chain Reaction
Messenger RNA

Keywords

  • Apoptosis
  • Ethanol
  • Puerariae flos

ASJC Scopus subject areas

  • Pharmacology
  • Molecular Medicine

Cite this

Protective effects of Puerariae flos against ethanol-induced apoptosis on human neuroblastoma cell line SK-N-MC. / Jang, Mi-Hyeon D; Shin, Min Chul; Kim, Youn Jung; Chung, Joo Ho; Yim, Sung Vin; Kim, Ee Hwa; Kim, Yeunhee; Kim, Chang Ju.

In: Japanese Journal of Pharmacology, Vol. 87, No. 4, 2001, p. 338-342.

Research output: Contribution to journalArticle

Jang, Mi-Hyeon D ; Shin, Min Chul ; Kim, Youn Jung ; Chung, Joo Ho ; Yim, Sung Vin ; Kim, Ee Hwa ; Kim, Yeunhee ; Kim, Chang Ju. / Protective effects of Puerariae flos against ethanol-induced apoptosis on human neuroblastoma cell line SK-N-MC. In: Japanese Journal of Pharmacology. 2001 ; Vol. 87, No. 4. pp. 338-342.
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