Protective effects of Puerariae flos against ethanol-induced apoptosis on human neuroblastoma cell line SK-N-MC

Mi Hyeon Jang, Min Chul Shin, Youn Jung Kim, Joo Ho Chung, Sung Vin Yim, Ee Hwa Kim, Yeunhee Kim, Chang Ju Kim

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Puerariae flos (PF) is an oriental medical herb for alcohol abuse. To investigate whether PF possesses protective effects against ethanol (EtOH)-induced cytotoxicity in the central nervous system, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, flow cytometric analysis, DNA fragmentation assay, and reverse transcription-polymerase chain reaction were performed on SK-N-MC human neuroblastoma cells. Cells treated with EtOH exhibited several apoptotic features, while those pre-treated with PF prior to EtOH exposure showed a decreased occurrence of apoptotic features. In addition, PF pre-treatment inhibited the EtOH-induced increase in caspase-3 mRNA expression. These results suggest that PF may exert protective effects against EtOH-induced apoptosis in human neuroblastoma cells.

Original languageEnglish (US)
Pages (from-to)338-342
Number of pages5
JournalJapanese Journal of Pharmacology
Volume87
Issue number4
DOIs
StatePublished - Dec 1 2001

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Keywords

  • Apoptosis
  • Ethanol
  • Puerariae flos

ASJC Scopus subject areas

  • Pharmacology

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