Protective effect of aqueous extract of Ginseng radix against 1-methyl-4-phenylpyridinium-induced apoptosis in PC12 cells

Ee Hwa Kim, Mi Hyeon Jang, Min Chul Shin, Mal Soon Shin, Chang Ju Kim

Research output: Contribution to journalArticle

40 Scopus citations

Abstract

Ginseng radix, the root of Panax ginseng C. A. MEYER (Araliaceae), is one of the best-known Oriental medicinal herbs with numerous therapeutic applications. To investigate whether Ginseng radix possesses a protective effect against 1-methyl-4-phenylpyridine (MPP+)-induced cytotoxicity in neuronal cells, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, flow cytometry, DNA fragmentation assay, reverse transcription-polymerase chain reaction (RT-PCR), Western blotting, and caspase-3 enzyme assay were performed on PC12 neuronal cells. Cells treated with MPP+ exhibited various apoptotic features, while cell pretreated with Ginseng radix prior to MPP+ exposure showed a decrease in the occurrence of apoptotic features. These results suggest that Ginseng radix may exert a protective effect against MPP+-induced apoptosis in PC12 cells.

Original languageEnglish (US)
Pages (from-to)1668-1673
Number of pages6
JournalBiological and Pharmaceutical Bulletin
Volume26
Issue number12
DOIs
StatePublished - Dec 1 2003

Keywords

  • 1-methyl-4-phenylpyridine (MPP )
  • Apoptosis
  • Ginseng radix
  • Parkinson's disease

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

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