Protective effect of Acanthopanax senticosus against ethanol-induced apoptosis of human neuroblastoma cell line SK-N-MC

Mi Hyeon Jang, Min Chul Shin, Youn Jung Kim, Chang Ju Kim, Joo Ho Chung, Jung Chul Seo, Ee Hwa Kim, Kyu Yeol Kim, Choong Yeol Lee, Kyeong Mi Kim

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

The protective effect of Acanthopanax senticosus (AS) against ethanol (EtOH)-induced apoptosis of the human neuroblastoma cell line SK-N-MC was investigated via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, flow cytometric analysis, DNA fragmentation assay, reverse transcription-polymerase chain reaction (RT-PCR), and caspase-3 assay. It was shown that cells treated with EtOH exhibit classical apoptotic features, while cells pre-treated with Acanthopanax senticosus prior to EtOH exposure showed decreased occurrence of apoptotic features. In addition, Acanthopanax senticosus pre-treatment was shown to inhibit EtOH-induced increase in caspase-3 mRNA expression and activity. These results suggest that Acanthopanax senticosus may exert a protective effect against EtOH-induced apoptosis of human neuroblastoma cells.

Original languageEnglish (US)
Pages (from-to)379-388
Number of pages10
JournalAmerican Journal of Chinese Medicine
Volume31
Issue number3
DOIs
StatePublished - 2003

Keywords

  • Acanthopanax senticosus
  • Apoptosis
  • Ethanol
  • Neuroblastoma Cell Line

ASJC Scopus subject areas

  • Complementary and alternative medicine

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