Abstract
The protective effect of Acanthopanax senticosus (AS) against ethanol (EtOH)-induced apoptosis of the human neuroblastoma cell line SK-N-MC was investigated via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, flow cytometric analysis, DNA fragmentation assay, reverse transcription-polymerase chain reaction (RT-PCR), and caspase-3 assay. It was shown that cells treated with EtOH exhibit classical apoptotic features, while cells pre-treated with Acanthopanax senticosus prior to EtOH exposure showed decreased occurrence of apoptotic features. In addition, Acanthopanax senticosus pre-treatment was shown to inhibit EtOH-induced increase in caspase-3 mRNA expression and activity. These results suggest that Acanthopanax senticosus may exert a protective effect against EtOH-induced apoptosis of human neuroblastoma cells.
Original language | English (US) |
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Pages (from-to) | 379-388 |
Number of pages | 10 |
Journal | American Journal of Chinese Medicine |
Volume | 31 |
Issue number | 3 |
DOIs | |
State | Published - 2003 |
Keywords
- Acanthopanax senticosus
- Apoptosis
- Ethanol
- Neuroblastoma Cell Line
ASJC Scopus subject areas
- Complementary and alternative medicine