Protective effect of Acanthopanax senticosus against ethanol-induced apoptosis of human neuroblastoma cell line SK-N-MC

Mi-Hyeon D Jang, Min Chul Shin, Youn Jung Kim, Chang Ju Kim, Joo Ho Chung, Jung Chul Seo, Ee Hwa Kim, Kyu Yeol Kim, Choong Yeol Lee, Kyeong Mi Kim

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The protective effect of Acanthopanax senticosus (AS) against ethanol (EtOH)-induced apoptosis of the human neuroblastoma cell line SK-N-MC was investigated via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, flow cytometric analysis, DNA fragmentation assay, reverse transcription-polymerase chain reaction (RT-PCR), and caspase-3 assay. It was shown that cells treated with EtOH exhibit classical apoptotic features, while cells pre-treated with Acanthopanax senticosus prior to EtOH exposure showed decreased occurrence of apoptotic features. In addition, Acanthopanax senticosus pre-treatment was shown to inhibit EtOH-induced increase in caspase-3 mRNA expression and activity. These results suggest that Acanthopanax senticosus may exert a protective effect against EtOH-induced apoptosis of human neuroblastoma cells.

Original languageEnglish (US)
Pages (from-to)379-388
Number of pages10
JournalAmerican Journal of Chinese Medicine
Volume31
Issue number3
DOIs
StatePublished - 2003
Externally publishedYes

Fingerprint

Eleutherococcus
Neuroblastoma
Ethanol
Apoptosis
Cell Line
Caspase 3
DNA Fragmentation
Reverse Transcription
Polymerase Chain Reaction
Messenger RNA

Keywords

  • Acanthopanax senticosus
  • Apoptosis
  • Ethanol
  • Neuroblastoma Cell Line

ASJC Scopus subject areas

  • Complementary and alternative medicine

Cite this

Protective effect of Acanthopanax senticosus against ethanol-induced apoptosis of human neuroblastoma cell line SK-N-MC. / Jang, Mi-Hyeon D; Shin, Min Chul; Kim, Youn Jung; Kim, Chang Ju; Chung, Joo Ho; Seo, Jung Chul; Kim, Ee Hwa; Kim, Kyu Yeol; Lee, Choong Yeol; Kim, Kyeong Mi.

In: American Journal of Chinese Medicine, Vol. 31, No. 3, 2003, p. 379-388.

Research output: Contribution to journalArticle

Jang, Mi-Hyeon D ; Shin, Min Chul ; Kim, Youn Jung ; Kim, Chang Ju ; Chung, Joo Ho ; Seo, Jung Chul ; Kim, Ee Hwa ; Kim, Kyu Yeol ; Lee, Choong Yeol ; Kim, Kyeong Mi. / Protective effect of Acanthopanax senticosus against ethanol-induced apoptosis of human neuroblastoma cell line SK-N-MC. In: American Journal of Chinese Medicine. 2003 ; Vol. 31, No. 3. pp. 379-388.
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