Prostatein C3‐mRNA: A sensitive marker of androgen‐responsiveness in prostate explant cultures

T. C. Shao, A. Kong, C. Young, W. H. Chu, D. J. Tindall, Glenn R. Cunningham

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Prostatein is an androgen‐dependent protein which is secreted by the rat ventral prostate. To determine if prostatein or its mRNA were responsive to androgen in vitro, prostate explants were cultured in media containing 0 or 25 nM dihydrotestosterone (DHT), estradiol (E2), or cortisol (F). Prostatein concentrations in medium were measured by radioimmunoassay at 2 and 4 days and in homogenates at 4 days. They were not changed significantly by any of these steroids. The concentration of the mRNA for the C3‐subunit of prostatein was determined by dot hybridization at 0, 2, 4, 6, and 8 days. It was decreased significantly by 2 days when compared with explants cultured in the presence of DHT and significant differences persisted through 8 days. In conclusion, quantitation of the mRNA for the C3‐subunit of prostatein in short‐term cultures of ventral prostate explants appears to be more sensitive to changes in androgen concentration than does measurement of prostatein, per se. Prostatein C3‐mRNA may be a useful marker for in vitro studies of androgen agonists and antagonists.

Original languageEnglish (US)
Pages (from-to)41-55
Number of pages15
JournalThe Prostate
Volume17
Issue number1
DOIs
StatePublished - 1990

Keywords

  • androgen
  • prostatein
  • rat ventral prostate

ASJC Scopus subject areas

  • Oncology
  • Urology

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    Shao, T. C., Kong, A., Young, C., Chu, W. H., Tindall, D. J., & Cunningham, G. R. (1990). Prostatein C3‐mRNA: A sensitive marker of androgen‐responsiveness in prostate explant cultures. The Prostate, 17(1), 41-55. https://doi.org/10.1002/pros.2990170106