TY - JOUR
T1 - Prostaglandin F2α initiates polyphosphatidylinositol hydrolysis and membrane translocation of protein kinase C in swine ovarian cells
AU - Veldhuis, Johannes D.
PY - 1987/11/30
Y1 - 1987/11/30
N2 - The biochemical mechanisms subserving the inhibitory actions of prostaglandin F2α on ovarian cells are not known. Since the protein kinase C pathway is coupled to steroidogenesis in an inhibitory fashion in pig granulosa cells, we have tested the hypothesis that prostaglandin F2α activates this phospholipid-dependent, calcium-stimulated effector pathway. Using monolayer cultures of swine granulosa cells, we now report that prostaglandin F2α is capable of activating critical components of the protein kinase C pathway, including the production of water-soluble inositol phosphates, liberation of free arachidonic acid, release of endogenous diacylglycerol, and translocation of cytosolic protein kinase C to the phospholipid-enriched membrane microenvironment.
AB - The biochemical mechanisms subserving the inhibitory actions of prostaglandin F2α on ovarian cells are not known. Since the protein kinase C pathway is coupled to steroidogenesis in an inhibitory fashion in pig granulosa cells, we have tested the hypothesis that prostaglandin F2α activates this phospholipid-dependent, calcium-stimulated effector pathway. Using monolayer cultures of swine granulosa cells, we now report that prostaglandin F2α is capable of activating critical components of the protein kinase C pathway, including the production of water-soluble inositol phosphates, liberation of free arachidonic acid, release of endogenous diacylglycerol, and translocation of cytosolic protein kinase C to the phospholipid-enriched membrane microenvironment.
UR - http://www.scopus.com/inward/record.url?scp=0023158981&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023158981&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(87)91611-1
DO - 10.1016/0006-291X(87)91611-1
M3 - Article
C2 - 3120721
AN - SCOPUS:0023158981
SN - 0006-291X
VL - 149
SP - 112
EP - 117
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -