Prostaglandin F initiates polyphosphatidylinositol hydrolysis and membrane translocation of protein kinase C in swine ovarian cells

Johannes D Veldhuis

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The biochemical mechanisms subserving the inhibitory actions of prostaglandin F on ovarian cells are not known. Since the protein kinase C pathway is coupled to steroidogenesis in an inhibitory fashion in pig granulosa cells, we have tested the hypothesis that prostaglandin F activates this phospholipid-dependent, calcium-stimulated effector pathway. Using monolayer cultures of swine granulosa cells, we now report that prostaglandin F is capable of activating critical components of the protein kinase C pathway, including the production of water-soluble inositol phosphates, liberation of free arachidonic acid, release of endogenous diacylglycerol, and translocation of cytosolic protein kinase C to the phospholipid-enriched membrane microenvironment.

Original languageEnglish (US)
Pages (from-to)112-117
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume149
Issue number1
DOIs
StatePublished - Nov 30 1987
Externally publishedYes

Fingerprint

Dinoprost
Protein Kinase C
Hydrolysis
Membrane Proteins
Swine
Granulosa Cells
Membranes
Phospholipids
Inositol Phosphates
Diglycerides
Arachidonic Acid
Monolayers
Calcium
Water

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

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AB - The biochemical mechanisms subserving the inhibitory actions of prostaglandin F2α on ovarian cells are not known. Since the protein kinase C pathway is coupled to steroidogenesis in an inhibitory fashion in pig granulosa cells, we have tested the hypothesis that prostaglandin F2α activates this phospholipid-dependent, calcium-stimulated effector pathway. Using monolayer cultures of swine granulosa cells, we now report that prostaglandin F2α is capable of activating critical components of the protein kinase C pathway, including the production of water-soluble inositol phosphates, liberation of free arachidonic acid, release of endogenous diacylglycerol, and translocation of cytosolic protein kinase C to the phospholipid-enriched membrane microenvironment.

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