Properties of WT and mutant hERG K+ channels expressed in neonatal mouse cardiomyocytes

Eric C. Lin, Katherine M. Holzem, Blake D. Anson, Brooke M. Moungey, Sadguna Y. Balijepalli, David J. Tester, Michael J. Ackerman, Brian P. Delisle, Ravi C. Balijepalli, Craig T. January

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Abstract

Mutations in human ether-a-go-go-related gene 1 (hERG) are linked to long QT syndrome type 2 (LQT2). hERG encodes the pore-forming α-subunits that coassemble to form rapidly activating delayed rectifier K+ current in the heart. LQT2-linked missense mutations have been extensively studied in noncardiac heterologous expression systems, where biogenic (protein trafficking) and biophysical (gating and permeation) abnormalities have been postulated to underlie the loss-of-function phenotype associated with LQT2 channels. Little is known about the properties of LQT2-linked hERG channel proteins in native cardiomyocyte systems. In this study, we expressed wild-type (WT) hERG and three LQT2-linked mutations in neonatal mouse cardiomyocytes and studied their electrophysiological and biochemical properties. Compared with WT hERG channels, the LQT2 missense mutations G601S and N470D hERG exhibited altered protein trafficking and underwent pharmacological correction, and N470D hERG channels gated at more negative voltages. The ΔY475 hERG deletion mutation trafficked similar to WT hERG channels, gated at more negative voltages, and had rapid deactivation kinetics, and these properties were confirmed in both neonatal mouse cardiomyocyte and human embryonic kidney (HEK)-293 cell expression systems. Differences between the cardiomyocytes and HEK-293 cell expression systems were that hERG current densities were reduced 10-fold and deactivation kinetics were accelerated 1.5- to 2-fold in neonatal mouse cardiomyocytes. An important finding of this work is that pharmacological correction of trafficking-deficient LQT2 mutations, as a potential innovative approach to therapy, is possible in native cardiac tissue.

Original languageEnglish (US)
Pages (from-to)H1842-H1849
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume298
Issue number6
DOIs
StatePublished - Jun 1 2010

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Keywords

  • E4031
  • Human ether-a-go-go-related gene 1
  • Long qt syndrome 2
  • Protein trafficking

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

Lin, E. C., Holzem, K. M., Anson, B. D., Moungey, B. M., Balijepalli, S. Y., Tester, D. J., Ackerman, M. J., Delisle, B. P., Balijepalli, R. C., & January, C. T. (2010). Properties of WT and mutant hERG K+ channels expressed in neonatal mouse cardiomyocytes. American Journal of Physiology - Heart and Circulatory Physiology, 298(6), H1842-H1849. https://doi.org/10.1152/ajpheart.01236.2009