Properties of an intermediate-sized androgen receptor: Association with RNA

David R. Rowley, Richard T. Premont, Michael P. Johnson, Charles Y F Young, Donald J. Tindall

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

This study identifies an intermediate-sized androgen receptor and characterizes its relationship with the 9.1S and 4.4S receptor forms. Under low ionic conditions, at 2-4 °C, there exists a 9.1S (±0.17) (n = 30) oligomeric form which does not bind to DNA. Under high ionic conditions, this form dissociates to a 4.4S (±0.08) (n = 18) monomeric form. When the salt concentration is lowered, the 4.4S monomer converts to a species with an intermediate sedimentation coefficient of 7.7S (±0.15) (n -17) which binds to DNA. Unlike the 9.1S oligomer the 7.7S form is not maintained by sodium molybdate under high ionic conditions but rather dissociates to the 4.4S monomer. To determine whether these forms were associated with RNA, the 7.7S form was incubated with RNase A and analyzed by density gradient centrifugation. The 7.7S form was digested fully by RNase to the 4.4S monomer. The 7.7S form demonstrated a buoyant density of 1.2459 ± 0.014 g/cm3 (n = 6) in metrizamide gradients, suggesting a ribonucleoprotein component. The sedimentation coefficient of the 9.1S form was unaffected by RNase. These data suggest that the intermediate 7.7S receptor form is composed of 4.4S monomer associated with a ribonucleoprotein molecule(s).

Original languageEnglish (US)
Pages (from-to)6988-6995
Number of pages8
JournalBiochemistry®
Volume25
Issue number22
StatePublished - 1986
Externally publishedYes

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Ribonucleoproteins
Androgen Receptors
Ribonucleases
Monomers
RNA
Metrizamide
Pancreatic Ribonuclease
Density Gradient Centrifugation
DNA
Sedimentation
Salts
Centrifugation
Oligomers
Molecules
sodium molybdate(VI)

ASJC Scopus subject areas

  • Biochemistry

Cite this

Rowley, D. R., Premont, R. T., Johnson, M. P., Young, C. Y. F., & Tindall, D. J. (1986). Properties of an intermediate-sized androgen receptor: Association with RNA. Biochemistry®, 25(22), 6988-6995.

Properties of an intermediate-sized androgen receptor : Association with RNA. / Rowley, David R.; Premont, Richard T.; Johnson, Michael P.; Young, Charles Y F; Tindall, Donald J.

In: Biochemistry®, Vol. 25, No. 22, 1986, p. 6988-6995.

Research output: Contribution to journalArticle

Rowley, DR, Premont, RT, Johnson, MP, Young, CYF & Tindall, DJ 1986, 'Properties of an intermediate-sized androgen receptor: Association with RNA', Biochemistry®, vol. 25, no. 22, pp. 6988-6995.
Rowley DR, Premont RT, Johnson MP, Young CYF, Tindall DJ. Properties of an intermediate-sized androgen receptor: Association with RNA. Biochemistry®. 1986;25(22):6988-6995.
Rowley, David R. ; Premont, Richard T. ; Johnson, Michael P. ; Young, Charles Y F ; Tindall, Donald J. / Properties of an intermediate-sized androgen receptor : Association with RNA. In: Biochemistry®. 1986 ; Vol. 25, No. 22. pp. 6988-6995.
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