Properties of β-adrenergic receptors on porcine corpora lutea and granulosa cells

S. N. Perkins, M. J. Cronin, Johannes D Veldhuis

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Abstract

The nature of β-adrenergic binding by swine corpora lutea and granulosa cells was examined with the specific β-adrenergic radioligand, (±)3-[ 125I]iodocyanopindolol (ICYP). Saturation analyses revealed the presence of high affinity (K(d) = 15.2 ± 2.1 pM; n = 8 experiments) and low capacity (6.7 ± 0.8 fmol/mg protein) β-adrenergic receptors on porcine corpora lutea membranes. The properties of β-adrenergic binding were determined by computer modeling of competition studies with a variety of compounds selective for β-adrenergic subtypes. These studies disclosed predominantly β 1-adrenergic receptors on pig luteal membranes. This inference from radioligand binding studies was corroborated functionally by the approximately equipotent biological effects of L-norepinephrine and L-epinephrine on cAMP production by luteal tissue (respective EC 50s of 282 ± 31 and 187 ± 66 nM; n = 3 experiments). Physiological regulation of specific β-adrenergic receptor content in the swine ovary was indicated by prominent (up to 9-fold) variations in receptor concentrations among corpora lutea and granulosa cells at various stages of maturity. In addition, there was differential expression of β-adrenergic receptor subtype. Whereas the β-adrenergic receptor subtype was predominantly β 1 in corpora hemorrhagica and corpora lutea, granulosa cells and corpora albicantia contained principally β 2 receptors. This difference could not be accounted for by blood cell contamination of corpora lutea, since swine blood cells contained pr edominantly (>98%) β 2-receptors, which were present at less than 8.6% the concentration of total β-receptors in luteal tissue. In summary, swine corpora lutea and granulosa cells contain specific high affinity, low capacity β-adrenergic receptors that are functionally coupled to biological responses. Moreover, total receptor content as well as β-adrenergic subtype exhibit significant physiological variation in relation to maturational status of ovarian follicular and luteal tissue.

Original languageEnglish (US)
Pages (from-to)998-1005
Number of pages8
JournalEndocrinology
Volume118
Issue number3
StatePublished - 1986
Externally publishedYes

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ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Perkins, S. N., Cronin, M. J., & Veldhuis, J. D. (1986). Properties of β-adrenergic receptors on porcine corpora lutea and granulosa cells. Endocrinology, 118(3), 998-1005.