Progressive Fibrosis: A Progesterone-and KLF11-Mediated Sexually Dimorphic Female Response

Chandra C. Shenoy, Zaraq Khan, Ye Zheng, Tiffanny L. Jones, Khashayarsha Khazaie, Gaurang S. Daftary

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Progressive scarring is ubiquitous postoperatively and in an array of chronic systemic diseases. Recent studies indicate that such scarring has a high female propensity; females are also almost exclusively affected by endometriosis, a common sex steroid-dependent fibrotic disease. Endometriosis-related fibrosis is regulated epigenetically through transcription factor Krü ppel-like factor 11 (KLF11). In response to surgical induction of endometriosis, Klf112/2 female mice develop significant fibrosis in contrast to wild-Type mice. We therefore hypothesized that female fibrotic predilection was mediated by differential sex steroid regulation of KLF11/collagen 1a1 signaling and investigated the fibrotic response in wild-Type and Klf112/2 male and female animals using a sterile peritonitis model. Fibrosis selectively developed in Klf112/2 females. Fibrosis in these animals was almost completely abrogated by ovariectomy. Ovariectomized animals were selectively supplemented with estradiol, medroxyprogesterone acetate (MPA), or dihydrotestosterone; fibrosis was only observed in mice exposed to MPA. Fibrosis therefore selectively developed in Klf112/2 female mice in response to physiological or pharmacological progesterone. The fibrotic response in these animals was also mitigated in response to antiprogestin therapy. Profibrotic gene expression was activated in a primary human peritoneal cell line in response to KLF11 short hairpin RNA and MPA but not estradiol. KLF11/collagen 1a1 signaling previously shown to be linked to fibrosis was thus selectively dysregulated in MPA-Treated cells. Our in vivo and in vitro findings in an animal model and human cells, respectively, suggest that progressive fibrotic scarring is a sexually dimorphic response irrespective of etiology; moreover, it is responsive to novel, individualized therapeutic intervention.

Original languageEnglish (US)
Pages (from-to)3605-3619
Number of pages15
JournalEndocrinology
Volume158
Issue number10
DOIs
StatePublished - Oct 1 2017

ASJC Scopus subject areas

  • Endocrinology

Fingerprint Dive into the research topics of 'Progressive Fibrosis: A Progesterone-and KLF11-Mediated Sexually Dimorphic Female Response'. Together they form a unique fingerprint.

  • Cite this