Progressive depletion of peripheral B lymphocytes in 4-1BB (CD137) ligand/I-Eα-transgenic mice

G. Zhu, D. B. Flies, K. Tamada, Y. Sun, M. Rodriguez, Y. X. Fu, L. Chen

Research output: Contribution to journalArticle

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Abstract

Interaction of 4-1BB (CD137) and its ligand (4-1BBL) is thought to positively regulate cell-mediated and humoral immune responses. We have prepared transgenic mouse strains that express 4-1BBL cDNA under the control of MHC class II I-Eα promoter. The 4-1BBL-transgenic mice show progressive splenomegaly and selective depletion of B220+ B cells accompanied with low levels of circulating IgG and defective humoral responses to Ag challenge. In addition, splenocytes from the transgenic mice fail to provide stimulation for allogeneic T cells in both lymphoproliferative and CTL responses in vitro, whereas their T cells remain functionally normal. Our results reveal unexpected functions of 4-1BBL in the regulation of humoral immune responses and Ag presentation.

Original languageEnglish (US)
Pages (from-to)2671-2676
Number of pages6
JournalJournal of Immunology
Volume167
Issue number5
StatePublished - Sep 1 2001

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4-1BB Ligand
Transgenic Mice
B-Lymphocytes
Humoral Immunity
T-Lymphocytes
Splenomegaly
Complementary DNA
Immunoglobulin G

ASJC Scopus subject areas

  • Immunology

Cite this

Zhu, G., Flies, D. B., Tamada, K., Sun, Y., Rodriguez, M., Fu, Y. X., & Chen, L. (2001). Progressive depletion of peripheral B lymphocytes in 4-1BB (CD137) ligand/I-Eα-transgenic mice. Journal of Immunology, 167(5), 2671-2676.

Progressive depletion of peripheral B lymphocytes in 4-1BB (CD137) ligand/I-Eα-transgenic mice. / Zhu, G.; Flies, D. B.; Tamada, K.; Sun, Y.; Rodriguez, M.; Fu, Y. X.; Chen, L.

In: Journal of Immunology, Vol. 167, No. 5, 01.09.2001, p. 2671-2676.

Research output: Contribution to journalArticle

Zhu, G, Flies, DB, Tamada, K, Sun, Y, Rodriguez, M, Fu, YX & Chen, L 2001, 'Progressive depletion of peripheral B lymphocytes in 4-1BB (CD137) ligand/I-Eα-transgenic mice', Journal of Immunology, vol. 167, no. 5, pp. 2671-2676.
Zhu G, Flies DB, Tamada K, Sun Y, Rodriguez M, Fu YX et al. Progressive depletion of peripheral B lymphocytes in 4-1BB (CD137) ligand/I-Eα-transgenic mice. Journal of Immunology. 2001 Sep 1;167(5):2671-2676.
Zhu, G. ; Flies, D. B. ; Tamada, K. ; Sun, Y. ; Rodriguez, M. ; Fu, Y. X. ; Chen, L. / Progressive depletion of peripheral B lymphocytes in 4-1BB (CD137) ligand/I-Eα-transgenic mice. In: Journal of Immunology. 2001 ; Vol. 167, No. 5. pp. 2671-2676.
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