Progress toward isolation of a breast cancer susceptibility gene, BRCA1

B. L. Weber; K. J. Abel; F. J. Couch; S. D. Merajver; S. C. Chandrasekharappa; L. Castilla; D. McKinley; P. P. Ho; K. Calzone; T. S. Frank; J. Xu; L. C. Brody; F. S. Collins

doi:10.1101/SQB.1994.059.01.059

Progress toward isolation of a breast cancer susceptibility gene, BRCA1

B. L. Weber, K. J. Abel, F. J. Couch, S. D. Merajver, S. C. Chandrasekharappa, L. Castilla, D. McKinley, P. P. Ho, K. Calzone, T. S. Frank, J. Xu, L. C. Brody, F. S. Collins

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Abstract

The high incidence of breast cancer and/or ovarian cancer in some families appeals to be due to germ line mutations in BRCA1. Genetic analysis of such families suggests that the BRCA1 candidate region lies between D17S857 and D17S78 on chromosome 17q21 (Kelsell et at. 1993; Simard at al. 1993). To identify and isolate BRCA1, we have used linkage and meiotic recombination analysis, characterized regions displaying LOH in tumor DNA from BRCA1- linked families, performed YAC and cosmid clone isolation and ordering, and used three complementary transcript searching strategies. We have identified as many as 28 genes from the BRCA1 candidate region, and we are searching for constitutive mutations in these candidate genes by several methods in an attempt to identify BRCA1.

Original language	English (US)
Pages (from-to)	531-536
Number of pages	6
Journal	Cold Spring Harbor Symposia on Quantitative Biology
Volume	59
DOIs	https://doi.org/10.1101/SQB.1994.059.01.059
State	Published - 1994

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Genetics

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Weber, B. L., Abel, K. J., Couch, F. J., Merajver, S. D., Chandrasekharappa, S. C., Castilla, L., McKinley, D., Ho, P. P., Calzone, K., Frank, T. S., Xu, J., Brody, L. C., & Collins, F. S. (1994). Progress toward isolation of a breast cancer susceptibility gene, BRCA1. Cold Spring Harbor Symposia on Quantitative Biology, 59, 531-536. https://doi.org/10.1101/SQB.1994.059.01.059

@article{3d5eec276f5148ee8ab4703b443f5a63,

title = "Progress toward isolation of a breast cancer susceptibility gene, BRCA1",

abstract = "The high incidence of breast cancer and/or ovarian cancer in some families appeals to be due to germ line mutations in BRCA1. Genetic analysis of such families suggests that the BRCA1 candidate region lies between D17S857 and D17S78 on chromosome 17q21 (Kelsell et at. 1993; Simard at al. 1993). To identify and isolate BRCA1, we have used linkage and meiotic recombination analysis, characterized regions displaying LOH in tumor DNA from BRCA1- linked families, performed YAC and cosmid clone isolation and ordering, and used three complementary transcript searching strategies. We have identified as many as 28 genes from the BRCA1 candidate region, and we are searching for constitutive mutations in these candidate genes by several methods in an attempt to identify BRCA1.",

author = "Weber, {B. L.} and Abel, {K. J.} and Couch, {F. J.} and Merajver, {S. D.} and Chandrasekharappa, {S. C.} and L. Castilla and D. McKinley and Ho, {P. P.} and K. Calzone and Frank, {T. S.} and J. Xu and Brody, {L. C.} and Collins, {F. S.}",

year = "1994",

doi = "10.1101/SQB.1994.059.01.059",

language = "English (US)",

volume = "59",

pages = "531--536",

journal = "Cold Spring Harbor Symposia on Quantitative Biology",

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T1 - Progress toward isolation of a breast cancer susceptibility gene, BRCA1

AU - Weber, B. L.

AU - Abel, K. J.

AU - Couch, F. J.

AU - Merajver, S. D.

AU - Chandrasekharappa, S. C.

AU - Castilla, L.

AU - McKinley, D.

AU - Ho, P. P.

AU - Calzone, K.

AU - Frank, T. S.

AU - Xu, J.

AU - Brody, L. C.

AU - Collins, F. S.

PY - 1994

Y1 - 1994

N2 - The high incidence of breast cancer and/or ovarian cancer in some families appeals to be due to germ line mutations in BRCA1. Genetic analysis of such families suggests that the BRCA1 candidate region lies between D17S857 and D17S78 on chromosome 17q21 (Kelsell et at. 1993; Simard at al. 1993). To identify and isolate BRCA1, we have used linkage and meiotic recombination analysis, characterized regions displaying LOH in tumor DNA from BRCA1- linked families, performed YAC and cosmid clone isolation and ordering, and used three complementary transcript searching strategies. We have identified as many as 28 genes from the BRCA1 candidate region, and we are searching for constitutive mutations in these candidate genes by several methods in an attempt to identify BRCA1.

AB - The high incidence of breast cancer and/or ovarian cancer in some families appeals to be due to germ line mutations in BRCA1. Genetic analysis of such families suggests that the BRCA1 candidate region lies between D17S857 and D17S78 on chromosome 17q21 (Kelsell et at. 1993; Simard at al. 1993). To identify and isolate BRCA1, we have used linkage and meiotic recombination analysis, characterized regions displaying LOH in tumor DNA from BRCA1- linked families, performed YAC and cosmid clone isolation and ordering, and used three complementary transcript searching strategies. We have identified as many as 28 genes from the BRCA1 candidate region, and we are searching for constitutive mutations in these candidate genes by several methods in an attempt to identify BRCA1.

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Progress toward isolation of a breast cancer susceptibility gene, BRCA1

Abstract

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