Prognostic value of TP53 and K-ras-2 mutational analysis in stage III carcinoma of the colon

Victor E. Pricolo, Sydney D. Finkelstein, Tsung Teh Wu, Gerald Keller, Anke Bakker, Patricia A. Swalsky, Kirby I. Bland

Research output: Contribution to journalArticle

70 Scopus citations

Abstract

BACKGROUND: Genetic mutations involving oncogenes and tumor-suppressor genes occur in carcinogenesis, and may effect biologic behavior of neoplasms. In this study, we analyzed the prognostic value of mutational analysis in colon carcinoma. PATIENTS AND METHODS: Archival pathology specimens from 70 consecutive patients, resected for stage III colon carcinoma, were analyzed for point mutations by amplification and direct sequencing of exons from the K-ras-2 and the TP53 genes (topographic genotyping). Mutations were compared with adverse histopathologic features (poor differentiation, vascular and lymphatic invasion, mucin production) as prognostic markers. RESULTS: Five- year survival was 75% in patients with nonmutated lesions, significantly lower (21%) with TP53 mutations (P = 0.01), and intermediate with K-ras-2 only (45%) or both K-ras-2 and TP53 mutations (36%). A TP53 mutation carried the highest relative risk of death (2.39; 95% confidence interval, 1.29 to 4.42; P = 0.006). There was an additive effect on the risk of death between TP53 mutations and adverse histopathologic features. CONCLUSIONS: The information derived from mutational analysis is creating new prognostic variables that may play a role in the choice of therapy for colorectal carcinoma.

Original languageEnglish (US)
Pages (from-to)41-46
Number of pages6
JournalAmerican journal of surgery
Volume171
Issue number1
DOIs
StatePublished - Jan 1996

ASJC Scopus subject areas

  • Surgery

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    Pricolo, V. E., Finkelstein, S. D., Wu, T. T., Keller, G., Bakker, A., Swalsky, P. A., & Bland, K. I. (1996). Prognostic value of TP53 and K-ras-2 mutational analysis in stage III carcinoma of the colon. American journal of surgery, 171(1), 41-46. https://doi.org/10.1016/S0002-9610(99)80071-3