Prognostic significance of differentially expressed miRNAs in esophageal cancer

Yuxin Hu, Arlene M. Correa, Ashraful Hoque, Baoxiang Guan, Fei Ye, Jie Huang, Stephen G. Swisher, Tsung Teh Wu, Jaffer A. Ajani, Xiao Chun Xu

Research output: Contribution to journalArticle

135 Citations (Scopus)

Abstract

Altered microRNA (miRNA) expression has been found to promote carcinogenesis, but little is known about the role of miRNAs in esophageal cancer. In this study, we selected 10 miRNAs and analyzed their expression in 10 esophageal cancer cell lines and 158 tissue specimens using Northern blotting and in situ hybridization, respectively. We found that Let-7g, miR-21 and miR-195p were expressed in all 10 cell lines, miR-9 and miR-20a were not expressed in any of the cell lines, and miR-16-2, miR-30e, miR-34a, miR-126 and miR-200a were expressed in some of the cell lines but not others. In addition, transient transfection of miR-34a inhibited c-Met and cyclin D1 expression and esophageal cancer cell proliferation, whereas miR-16-2 suppressed RAR-β2 expression and increased tumor cell proliferation. Furthermore, we found that miR-126 expression was associated with tumor cell dedifferentiation and lymph node metastasis, miR-16-2 was associated with lymph node metastasis, and miR-195p was associated with higher pathologic disease stages in patients with esophageal adenocarcinoma. Kaplan-Meier analysis showed that miR-16-2 expression and miR-30e expression were associated with shorter overall and disease-free survival in all esophageal cancer patients. In addition, miR-16-2, miR-30e and miR-200a expression were associated with shorter overall and disease-free survival in patients with esophageal adenocarcinoma; however, miR-16-2, miR-30e and miR-200a expression were not associated with overall or disease-free survival in squamous cell carcinoma patients. Our data indicate that further evaluation of miR-30e and miR-16-2 as prognostic biomarkers is warranted in patients with esophageal adenocarcinoma. In addition, the role of miR-34a in esophageal cancer also warrants further study.

Original languageEnglish (US)
Pages (from-to)132-143
Number of pages12
JournalInternational Journal of Cancer
Volume128
Issue number1
DOIs
StatePublished - Jan 1 2011

Fingerprint

Esophageal Neoplasms
MicroRNAs
Disease-Free Survival
Cell Line
Adenocarcinoma
Lymph Nodes
Cell Dedifferentiation
Cell Proliferation
Neoplasm Metastasis
Cyclin D1
Kaplan-Meier Estimate
Northern Blotting
In Situ Hybridization
Transfection
Squamous Cell Carcinoma
Neoplasms
Carcinogenesis
Biomarkers

Keywords

  • Cell viability
  • Esophageal cancer
  • Gene regulation
  • MiRNA
  • Prognosis

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Hu, Y., Correa, A. M., Hoque, A., Guan, B., Ye, F., Huang, J., ... Xu, X. C. (2011). Prognostic significance of differentially expressed miRNAs in esophageal cancer. International Journal of Cancer, 128(1), 132-143. https://doi.org/10.1002/ijc.25330

Prognostic significance of differentially expressed miRNAs in esophageal cancer. / Hu, Yuxin; Correa, Arlene M.; Hoque, Ashraful; Guan, Baoxiang; Ye, Fei; Huang, Jie; Swisher, Stephen G.; Wu, Tsung Teh; Ajani, Jaffer A.; Xu, Xiao Chun.

In: International Journal of Cancer, Vol. 128, No. 1, 01.01.2011, p. 132-143.

Research output: Contribution to journalArticle

Hu, Y, Correa, AM, Hoque, A, Guan, B, Ye, F, Huang, J, Swisher, SG, Wu, TT, Ajani, JA & Xu, XC 2011, 'Prognostic significance of differentially expressed miRNAs in esophageal cancer', International Journal of Cancer, vol. 128, no. 1, pp. 132-143. https://doi.org/10.1002/ijc.25330
Hu, Yuxin ; Correa, Arlene M. ; Hoque, Ashraful ; Guan, Baoxiang ; Ye, Fei ; Huang, Jie ; Swisher, Stephen G. ; Wu, Tsung Teh ; Ajani, Jaffer A. ; Xu, Xiao Chun. / Prognostic significance of differentially expressed miRNAs in esophageal cancer. In: International Journal of Cancer. 2011 ; Vol. 128, No. 1. pp. 132-143.
@article{5bb774227225464a8dd6ea7634596065,
title = "Prognostic significance of differentially expressed miRNAs in esophageal cancer",
abstract = "Altered microRNA (miRNA) expression has been found to promote carcinogenesis, but little is known about the role of miRNAs in esophageal cancer. In this study, we selected 10 miRNAs and analyzed their expression in 10 esophageal cancer cell lines and 158 tissue specimens using Northern blotting and in situ hybridization, respectively. We found that Let-7g, miR-21 and miR-195p were expressed in all 10 cell lines, miR-9 and miR-20a were not expressed in any of the cell lines, and miR-16-2, miR-30e, miR-34a, miR-126 and miR-200a were expressed in some of the cell lines but not others. In addition, transient transfection of miR-34a inhibited c-Met and cyclin D1 expression and esophageal cancer cell proliferation, whereas miR-16-2 suppressed RAR-β2 expression and increased tumor cell proliferation. Furthermore, we found that miR-126 expression was associated with tumor cell dedifferentiation and lymph node metastasis, miR-16-2 was associated with lymph node metastasis, and miR-195p was associated with higher pathologic disease stages in patients with esophageal adenocarcinoma. Kaplan-Meier analysis showed that miR-16-2 expression and miR-30e expression were associated with shorter overall and disease-free survival in all esophageal cancer patients. In addition, miR-16-2, miR-30e and miR-200a expression were associated with shorter overall and disease-free survival in patients with esophageal adenocarcinoma; however, miR-16-2, miR-30e and miR-200a expression were not associated with overall or disease-free survival in squamous cell carcinoma patients. Our data indicate that further evaluation of miR-30e and miR-16-2 as prognostic biomarkers is warranted in patients with esophageal adenocarcinoma. In addition, the role of miR-34a in esophageal cancer also warrants further study.",
keywords = "Cell viability, Esophageal cancer, Gene regulation, MiRNA, Prognosis",
author = "Yuxin Hu and Correa, {Arlene M.} and Ashraful Hoque and Baoxiang Guan and Fei Ye and Jie Huang and Swisher, {Stephen G.} and Wu, {Tsung Teh} and Ajani, {Jaffer A.} and Xu, {Xiao Chun}",
year = "2011",
month = "1",
day = "1",
doi = "10.1002/ijc.25330",
language = "English (US)",
volume = "128",
pages = "132--143",
journal = "International Journal of Cancer",
issn = "0020-7136",
publisher = "Wiley-Liss Inc.",
number = "1",

}

TY - JOUR

T1 - Prognostic significance of differentially expressed miRNAs in esophageal cancer

AU - Hu, Yuxin

AU - Correa, Arlene M.

AU - Hoque, Ashraful

AU - Guan, Baoxiang

AU - Ye, Fei

AU - Huang, Jie

AU - Swisher, Stephen G.

AU - Wu, Tsung Teh

AU - Ajani, Jaffer A.

AU - Xu, Xiao Chun

PY - 2011/1/1

Y1 - 2011/1/1

N2 - Altered microRNA (miRNA) expression has been found to promote carcinogenesis, but little is known about the role of miRNAs in esophageal cancer. In this study, we selected 10 miRNAs and analyzed their expression in 10 esophageal cancer cell lines and 158 tissue specimens using Northern blotting and in situ hybridization, respectively. We found that Let-7g, miR-21 and miR-195p were expressed in all 10 cell lines, miR-9 and miR-20a were not expressed in any of the cell lines, and miR-16-2, miR-30e, miR-34a, miR-126 and miR-200a were expressed in some of the cell lines but not others. In addition, transient transfection of miR-34a inhibited c-Met and cyclin D1 expression and esophageal cancer cell proliferation, whereas miR-16-2 suppressed RAR-β2 expression and increased tumor cell proliferation. Furthermore, we found that miR-126 expression was associated with tumor cell dedifferentiation and lymph node metastasis, miR-16-2 was associated with lymph node metastasis, and miR-195p was associated with higher pathologic disease stages in patients with esophageal adenocarcinoma. Kaplan-Meier analysis showed that miR-16-2 expression and miR-30e expression were associated with shorter overall and disease-free survival in all esophageal cancer patients. In addition, miR-16-2, miR-30e and miR-200a expression were associated with shorter overall and disease-free survival in patients with esophageal adenocarcinoma; however, miR-16-2, miR-30e and miR-200a expression were not associated with overall or disease-free survival in squamous cell carcinoma patients. Our data indicate that further evaluation of miR-30e and miR-16-2 as prognostic biomarkers is warranted in patients with esophageal adenocarcinoma. In addition, the role of miR-34a in esophageal cancer also warrants further study.

AB - Altered microRNA (miRNA) expression has been found to promote carcinogenesis, but little is known about the role of miRNAs in esophageal cancer. In this study, we selected 10 miRNAs and analyzed their expression in 10 esophageal cancer cell lines and 158 tissue specimens using Northern blotting and in situ hybridization, respectively. We found that Let-7g, miR-21 and miR-195p were expressed in all 10 cell lines, miR-9 and miR-20a were not expressed in any of the cell lines, and miR-16-2, miR-30e, miR-34a, miR-126 and miR-200a were expressed in some of the cell lines but not others. In addition, transient transfection of miR-34a inhibited c-Met and cyclin D1 expression and esophageal cancer cell proliferation, whereas miR-16-2 suppressed RAR-β2 expression and increased tumor cell proliferation. Furthermore, we found that miR-126 expression was associated with tumor cell dedifferentiation and lymph node metastasis, miR-16-2 was associated with lymph node metastasis, and miR-195p was associated with higher pathologic disease stages in patients with esophageal adenocarcinoma. Kaplan-Meier analysis showed that miR-16-2 expression and miR-30e expression were associated with shorter overall and disease-free survival in all esophageal cancer patients. In addition, miR-16-2, miR-30e and miR-200a expression were associated with shorter overall and disease-free survival in patients with esophageal adenocarcinoma; however, miR-16-2, miR-30e and miR-200a expression were not associated with overall or disease-free survival in squamous cell carcinoma patients. Our data indicate that further evaluation of miR-30e and miR-16-2 as prognostic biomarkers is warranted in patients with esophageal adenocarcinoma. In addition, the role of miR-34a in esophageal cancer also warrants further study.

KW - Cell viability

KW - Esophageal cancer

KW - Gene regulation

KW - MiRNA

KW - Prognosis

UR - http://www.scopus.com/inward/record.url?scp=78349253777&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78349253777&partnerID=8YFLogxK

U2 - 10.1002/ijc.25330

DO - 10.1002/ijc.25330

M3 - Article

VL - 128

SP - 132

EP - 143

JO - International Journal of Cancer

JF - International Journal of Cancer

SN - 0020-7136

IS - 1

ER -