Progesterone binding to hen oviduct genome

Specific versus nonspecific binding

T. C. Spelsberg, G. M. Pikler, R. A. Webster

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Data are presented to explain discrepancies in the literature involving the in vitro binding of steroid receptor complexes to isolated nuclei and chromatin. The type of binding in vitro of the progesterone receptor complex to nuclei, chromatin, or DNA of hen organs is largely determined by the ionic strength of the medium. Low ionic conditions (0.01 to 0.05 molar potassium chloride) result in a nonspecific, nonsaturable binding, while high ionic c4nditions (0.15 to 0.20 molar potassium chloride) create a tissue specific, saturable binding. Pure DNA binds the steroid receptor complex extensively in low salt but very little in the higher salt conditions.

Original languageEnglish (US)
Pages (from-to)197-199
Number of pages3
JournalScience
Volume194
Issue number4261
StatePublished - 1976

Fingerprint

Oviducts
Potassium Chloride
Steroid Receptors
Chromatin
Progesterone
Salts
Genome
DNA
Progesterone Receptors
Osmolar Concentration
In Vitro Techniques

ASJC Scopus subject areas

  • General

Cite this

Spelsberg, T. C., Pikler, G. M., & Webster, R. A. (1976). Progesterone binding to hen oviduct genome: Specific versus nonspecific binding. Science, 194(4261), 197-199.

Progesterone binding to hen oviduct genome : Specific versus nonspecific binding. / Spelsberg, T. C.; Pikler, G. M.; Webster, R. A.

In: Science, Vol. 194, No. 4261, 1976, p. 197-199.

Research output: Contribution to journalArticle

Spelsberg, TC, Pikler, GM & Webster, RA 1976, 'Progesterone binding to hen oviduct genome: Specific versus nonspecific binding', Science, vol. 194, no. 4261, pp. 197-199.
Spelsberg TC, Pikler GM, Webster RA. Progesterone binding to hen oviduct genome: Specific versus nonspecific binding. Science. 1976;194(4261):197-199.
Spelsberg, T. C. ; Pikler, G. M. ; Webster, R. A. / Progesterone binding to hen oviduct genome : Specific versus nonspecific binding. In: Science. 1976 ; Vol. 194, No. 4261. pp. 197-199.
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