TY - JOUR
T1 - Progenitors of Interstitial Cells of Cajal in the Postnatal Murine Stomach
AU - Lorincz, Andrea
AU - Redelman, Doug
AU - Horváth, Viktor J.
AU - Bardsley, Michael R.
AU - Chen, Hui
AU - Ördög, Tamás
N1 - Funding Information:
Supported in part by National Institutes of Health grant DK58185. The Tissue Culture, Molecular and Morphology Core Laboratories were supported by National Institutes of Health grant DK41315. The University of Nevada, Reno Cytometry Center was supported in part by Nevada Biomedical Research Infrastructure Network grant RR16464 from the National Institutes of Health.
PY - 2008/4
Y1 - 2008/4
N2 - Background & Aims: Maintaining the integrity of networks of interstitial cells of Cajal (ICC) is essential to preserve orderly contractile activity and neuroregulation in the gastrointestinal tract and to restore these functions after tissue damage or surgeries. Maintenance of ICC requires insulin-dependent or insulin-like growth factor I (IGF-I)-dependent production of membrane-bound stem cell factor (SCF) and may involve regeneration from local progenitors. Our goal was to identify ICC precursors in postnatal murine gastric muscles. Methods: We used flow cytometry and immunohistochemistry to examine freshly dissected and cultured muscles for cells expressing CD34, an adhesion molecule expressed by stromal tumors; CD44, which occurs on mesenchymal stem cells; and receptors for SCF (Kit), insulin (Insr), and IGF-I (Igf1r). Slow waves were studied by intracellular recording. Results: In gastric muscles, we identified rare, KitlowCD44+CD34+Insr+Igf1r+ cells resembling common embryonic precursors of ICC and smooth muscle. These putative progenitors were absent from organotypic cultures lacking mature ICC (Kit+CD44+CD34-Insr-Igf1r-) due to prolonged insulin/IGF-I deprivation but were rescued by IGF-I that also prevented ICC loss. Soluble SCF failed to prevent the loss of mature ICC but dramatically expanded the putative progenitors, which supported robust slow wave activity despite retaining an immature, Kit+CD44+CD34+Insr+Igf1r+ phenotype. Differentiation of these cells into mature, network-forming ICC required IGF-I. Conversely, restoration of ICC networks by IGF-I after prolonged insulin and IGF-I deprivation required the survival of the presumed progenitors. Conclusions: KitlowCD44+CD34+Insr+Igf1r+ cells may be local progenitors for gastric ICC and stromal tumors. Loss of these cells may contribute to gastrointestinal dysmotilities.
AB - Background & Aims: Maintaining the integrity of networks of interstitial cells of Cajal (ICC) is essential to preserve orderly contractile activity and neuroregulation in the gastrointestinal tract and to restore these functions after tissue damage or surgeries. Maintenance of ICC requires insulin-dependent or insulin-like growth factor I (IGF-I)-dependent production of membrane-bound stem cell factor (SCF) and may involve regeneration from local progenitors. Our goal was to identify ICC precursors in postnatal murine gastric muscles. Methods: We used flow cytometry and immunohistochemistry to examine freshly dissected and cultured muscles for cells expressing CD34, an adhesion molecule expressed by stromal tumors; CD44, which occurs on mesenchymal stem cells; and receptors for SCF (Kit), insulin (Insr), and IGF-I (Igf1r). Slow waves were studied by intracellular recording. Results: In gastric muscles, we identified rare, KitlowCD44+CD34+Insr+Igf1r+ cells resembling common embryonic precursors of ICC and smooth muscle. These putative progenitors were absent from organotypic cultures lacking mature ICC (Kit+CD44+CD34-Insr-Igf1r-) due to prolonged insulin/IGF-I deprivation but were rescued by IGF-I that also prevented ICC loss. Soluble SCF failed to prevent the loss of mature ICC but dramatically expanded the putative progenitors, which supported robust slow wave activity despite retaining an immature, Kit+CD44+CD34+Insr+Igf1r+ phenotype. Differentiation of these cells into mature, network-forming ICC required IGF-I. Conversely, restoration of ICC networks by IGF-I after prolonged insulin and IGF-I deprivation required the survival of the presumed progenitors. Conclusions: KitlowCD44+CD34+Insr+Igf1r+ cells may be local progenitors for gastric ICC and stromal tumors. Loss of these cells may contribute to gastrointestinal dysmotilities.
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U2 - 10.1053/j.gastro.2008.01.036
DO - 10.1053/j.gastro.2008.01.036
M3 - Article
C2 - 18395089
AN - SCOPUS:41349105253
SN - 0016-5085
VL - 134
SP - 1083
EP - 1093
JO - Gastroenterology
JF - Gastroenterology
IS - 4
ER -