Production of the gaseous signal molecule hydrogen sulfide in mouse tissues

David R. Linden, Lei Sha, Amelia Mazzone, Gary J. Stoltz, Cheryl E. Bernard, Julie K. Furne, Michael D. Levitt, Gianrico Farrugia, Joseph H. Szurszewski

Research output: Contribution to journalArticle

114 Scopus citations

Abstract

The gaseous molecule hydrogen sulfide (H2S) has been proposed as an endogenous signal molecule and neuromodulator in mammals. Using a newly developed method, we report here for the first time the ability of intact and living brain and colonic tissue in the mouse to generate and release H 2S. This production occurs through the activity of two enzymes, cystathionine-γ-lyase and cystathionine-β-synthase. The quantitative expression of messenger RNA and protein localization for both enzymes are described in the liver, brain, and colon. Expression levels of the enzymes vary between tissues and are differentially distributed. The observation that, tissues that respond to exogenously applied H2S can endogenously generate the gas, strongly supports its role as an endogenous signal molecule.

Original languageEnglish (US)
Pages (from-to)1577-1585
Number of pages9
JournalJournal of neurochemistry
Volume106
Issue number4
DOIs
StatePublished - Aug 1 2008

Keywords

  • Aseptic dissection
  • Cysteine catabolism
  • Gastrointestinal
  • Immunohistochemistry
  • Trans-sulfuration

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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  • Cite this

    Linden, D. R., Sha, L., Mazzone, A., Stoltz, G. J., Bernard, C. E., Furne, J. K., Levitt, M. D., Farrugia, G., & Szurszewski, J. H. (2008). Production of the gaseous signal molecule hydrogen sulfide in mouse tissues. Journal of neurochemistry, 106(4), 1577-1585. https://doi.org/10.1111/j.1471-4159.2008.05502.x