Production of the gaseous signal molecule hydrogen sulfide in mouse tissues

David R. Linden; Lei Sha; Amelia Mazzone; Gary J. Stoltz; Cheryl E. Bernard; Julie K. Furne; Michael D. Levitt; Gianrico Farrugia; Joseph H. Szurszewski

doi:10.1111/j.1471-4159.2008.05502.x

Production of the gaseous signal molecule hydrogen sulfide in mouse tissues

David R. Linden, Lei Sha, Amelia Mazzone, Gary J. Stoltz, Cheryl E. Bernard, Julie K. Furne, Michael D. Levitt, Gianrico Farrugia, Joseph H. Szurszewski

Research output: Contribution to journal › Article › peer-review

118 Scopus citations

Abstract

The gaseous molecule hydrogen sulfide (H₂S) has been proposed as an endogenous signal molecule and neuromodulator in mammals. Using a newly developed method, we report here for the first time the ability of intact and living brain and colonic tissue in the mouse to generate and release H ₂S. This production occurs through the activity of two enzymes, cystathionine-γ-lyase and cystathionine-β-synthase. The quantitative expression of messenger RNA and protein localization for both enzymes are described in the liver, brain, and colon. Expression levels of the enzymes vary between tissues and are differentially distributed. The observation that, tissues that respond to exogenously applied H₂S can endogenously generate the gas, strongly supports its role as an endogenous signal molecule.

Original language	English (US)
Pages (from-to)	1577-1585
Number of pages	9
Journal	Journal of neurochemistry
Volume	106
Issue number	4
DOIs	https://doi.org/10.1111/j.1471-4159.2008.05502.x
State	Published - Aug 2008

Keywords

Aseptic dissection
Cysteine catabolism
Gastrointestinal
Immunohistochemistry
Trans-sulfuration

ASJC Scopus subject areas

Biochemistry
Cellular and Molecular Neuroscience

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10.1111/j.1471-4159.2008.05502.x

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title = "Production of the gaseous signal molecule hydrogen sulfide in mouse tissues",

abstract = "The gaseous molecule hydrogen sulfide (H2S) has been proposed as an endogenous signal molecule and neuromodulator in mammals. Using a newly developed method, we report here for the first time the ability of intact and living brain and colonic tissue in the mouse to generate and release H 2S. This production occurs through the activity of two enzymes, cystathionine-γ-lyase and cystathionine-β-synthase. The quantitative expression of messenger RNA and protein localization for both enzymes are described in the liver, brain, and colon. Expression levels of the enzymes vary between tissues and are differentially distributed. The observation that, tissues that respond to exogenously applied H2S can endogenously generate the gas, strongly supports its role as an endogenous signal molecule.",

keywords = "Aseptic dissection, Cysteine catabolism, Gastrointestinal, Immunohistochemistry, Trans-sulfuration",

author = "Linden, {David R.} and Lei Sha and Amelia Mazzone and Stoltz, {Gary J.} and Bernard, {Cheryl E.} and Furne, {Julie K.} and Levitt, {Michael D.} and Gianrico Farrugia and Szurszewski, {Joseph H.}",

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AU - Linden, David R.

AU - Sha, Lei

AU - Mazzone, Amelia

AU - Stoltz, Gary J.

AU - Bernard, Cheryl E.

AU - Furne, Julie K.

AU - Levitt, Michael D.

AU - Farrugia, Gianrico

AU - Szurszewski, Joseph H.

PY - 2008/8

Y1 - 2008/8

N2 - The gaseous molecule hydrogen sulfide (H2S) has been proposed as an endogenous signal molecule and neuromodulator in mammals. Using a newly developed method, we report here for the first time the ability of intact and living brain and colonic tissue in the mouse to generate and release H 2S. This production occurs through the activity of two enzymes, cystathionine-γ-lyase and cystathionine-β-synthase. The quantitative expression of messenger RNA and protein localization for both enzymes are described in the liver, brain, and colon. Expression levels of the enzymes vary between tissues and are differentially distributed. The observation that, tissues that respond to exogenously applied H2S can endogenously generate the gas, strongly supports its role as an endogenous signal molecule.

AB - The gaseous molecule hydrogen sulfide (H2S) has been proposed as an endogenous signal molecule and neuromodulator in mammals. Using a newly developed method, we report here for the first time the ability of intact and living brain and colonic tissue in the mouse to generate and release H 2S. This production occurs through the activity of two enzymes, cystathionine-γ-lyase and cystathionine-β-synthase. The quantitative expression of messenger RNA and protein localization for both enzymes are described in the liver, brain, and colon. Expression levels of the enzymes vary between tissues and are differentially distributed. The observation that, tissues that respond to exogenously applied H2S can endogenously generate the gas, strongly supports its role as an endogenous signal molecule.

KW - Aseptic dissection

KW - Cysteine catabolism

KW - Gastrointestinal

KW - Immunohistochemistry

KW - Trans-sulfuration

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JO - Journal of neurochemistry

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IS - 4

ER -

Production of the gaseous signal molecule hydrogen sulfide in mouse tissues

Abstract

Keywords

ASJC Scopus subject areas

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