Production of monoclonal antibodies by genetic immunization

Michael A Barry, M. E. Barry, S. A. Johnston

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Genetic immunization is a simple method for producing polyclonal antibodies in mice. To test if this approach could be used for monoclonal antibody production, biolistic transfection was used to immunize a mouse. High levels of polyclonal antibodies against human growth hormone (hGH) were elicited following three inoculations with the gene for hGH. When hybridoma cells were created from the mouse's spleenocytes, approximately 17% secreted antibodies vs. hGH. Of these, some recognized only native or denatured hGH, while most recognized both forms of the protein. These findings demonstrate the utility of genetic immunization as a method to produce monoclonal antibodies.

Original languageEnglish (US)
JournalBioTechniques
Volume16
Issue number4
StatePublished - 1994
Externally publishedYes

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Immunization
Human Growth Hormone
Monoclonal Antibodies
Antibodies
Biolistics
Hybridomas
Antibody Formation
Transfection
Genes
Proteins

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Clinical Biochemistry

Cite this

Production of monoclonal antibodies by genetic immunization. / Barry, Michael A; Barry, M. E.; Johnston, S. A.

In: BioTechniques, Vol. 16, No. 4, 1994.

Research output: Contribution to journalArticle

Barry, MA, Barry, ME & Johnston, SA 1994, 'Production of monoclonal antibodies by genetic immunization', BioTechniques, vol. 16, no. 4.
Barry, Michael A ; Barry, M. E. ; Johnston, S. A. / Production of monoclonal antibodies by genetic immunization. In: BioTechniques. 1994 ; Vol. 16, No. 4.
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